[Histonet] Treatment of calcified human arteries

[Elizabeth Chlipala]

Galina

I would decal in 5-10% formic acid that should work just fine for IHC staining.  You would fix, decal and then process.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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(303) 881-0763 cell
liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Galina Deyneko
Sent: Friday, February 14, 2014 9:06 AM
To: histology; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Treatment of calcified human arteries

Dear Colleagues
 Please share your expertise on treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining.
 Thank you in advance

Galina Deyneko
Novartis, Cambridge, MA
 
617-871-7613 w
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