[Histonet] Treatment of calcified human arteries

[Galina Deyneko]

Dear Colleagues
 Please share your expertise on treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining.
 Thank you in advance

Galina Deyneko
Novartis, Cambridge, MA
 
617-871-7613 w