[Histonet] Neg Ctls, Cryostat Decon, Tricrome stain and more

[Terri Braud]

Wow!  Three things!  

Negative IHC Controls - Since we use a polymer detection system, we long
ago quit using negative controls as a cost savings measure.  I can't
imagine doing anything less considering the cost.  It has saved us
thousands.

UV cryostat decontamination - Unfortunately, if you are a CAP accredited
lab, they do not recognize this as appropriate decontamination, but
insist on a room temperature decontamination, (ANP.12087 "all exposed
surfaces must be wiped with an EPA approved tuberculocidal
disinfectant") We use these really handy pop up disinfectant wipes that
are alcohol based, taking down one instrument for 48 hours to completely
dry at room temp before wiping and restarting.
I was told that CAP is looking into altering this based on differing
disinfection systems, such as UV, but until then, there is no
alternative if you are CAP

Trichrome stain - Just switch to the Gomori's one step with Aniline
Blue.  Much easier, fewer steps, almost goof proof. 


Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

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