[Histonet] RE: Buffer pH monitoring

[joelle weaver]

Same here. Each new bottle ( whether new lot or not, interpret as "batch"). Record pH on a QC sheet. It is nearly always in range being proprietary, unless user/maker error.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: thigginsht <@t> msn.com
> To: histonet <@t> lists.utsouthwestern.edu; ngupta1 <@t> hfhs.org
> Date: Tue, 11 Feb 2014 12:54:01 -0600
> CC: 
> Subject: [Histonet] RE: Buffer pH monitoring
> 
> I check pH every time I make a new batch(bottle) and then periodically there after. 
> 
> Timothy N. Higgins, HT (ASCP), QIHC
>  
> 
> > Message: 10
> > Date: Tue, 11 Feb 2014 16:23:22 +0000
> > From: "Gupta, Nilesh" <NGUPTA1 <@t> hfhs.org>
> > Subject: [Histonet] Buffer pH monitoring
> > To: "'histonet <@t> lists.utsouthwestern.edu'"
> > 	<histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > 	<F06DAF0DBFC4EC4C97B930056A759D5E247BFE12 <@t> RHWMBX15.corp.ds.hfhs.org>
> > Content-Type: text/plain; charset="utf-8"
> > 
> > I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer.
> > 
> > It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI
> > 
> > The pH of the buffers used in immunohistochemistry is routinely monitored.
> > 
> > NOTE:  pH must be tested when a new batch is prepared or received.
> > 
> > Evidence of Compliance:
> > 
> > ✓      Written procedure defining pH range for each buffer in use AND
> > 
> > ✓      Records of initial and subsequent QC on each buffer
> > 
> > 
> > 
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