[Histonet] RE: Buffer pH monitoring

[Tim Higgins]

I check pH every time I make a new batch(bottle) and then periodically there after. 

Timothy N. Higgins, HT (ASCP), QIHC
 

> Message: 10
> Date: Tue, 11 Feb 2014 16:23:22 +0000
> From: "Gupta, Nilesh" <NGUPTA1 <@t> hfhs.org>
> Subject: [Histonet] Buffer pH monitoring
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> 	<histonet <@t> lists.utsouthwestern.edu>
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> I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer.
> 
> It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI
> 
> The pH of the buffers used in immunohistochemistry is routinely monitored.
> 
> NOTE:  pH must be tested when a new batch is prepared or received.
> 
> Evidence of Compliance:
> 
> ✓      Written procedure defining pH range for each buffer in use AND
> 
> ✓      Records of initial and subsequent QC on each buffer
> 
> 
> 
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