[Histonet] Purple haze on H&E slides

[Burrage, Shannon L.]

Are you using acid alcohol, and Harris Hematoxylin? Are you filtering the heamatatoxylin every day? We still use a very small amount of gelatin in the waerbath. And get no background stain. So many veriables here.
Good luck,
Shannon Burrage 
shannon.burrage <@t> unitypoint.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Campbell, Tasha M. [tmcampbell <@t> fmh.org]
Sent: Monday, February 10, 2014 7:37 PM
To: Huggins, Haley - MRMC
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Purple haze on H&E slides

What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath?

Tasha

Sent from my iPhone

> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org> wrote:
>
> I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world.
>
> Haley Huggins
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> Histonet <@t> lists.utsouthwestern.edu
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