[Histonet] Purple haze on H&E slides

Campbell, Tasha M. tmcampbell <@t> fmh.org
Mon Feb 10 19:37:37 CST 2014


What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath?

Tasha

Sent from my iPhone

> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org> wrote:
> 
> I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world.
> 
> Haley Huggins
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