[Histonet] FoxP3 and Geminin Immunohistochemistry

[Troutman, Kenneth A]

Hello Steven,

I have used both of these antibodies for various projects and we performed all of these on the Bondmax from Leica with a 60 minute antibody incubation.

We have used two FoxP3 antibodies from eBiosciences; cat# 14-4777 on human tissue and cat# 13-5773 for mouse tissue.  (both were 1:100 dilutions and ER2 for 20 mins (EDTA pH9).

I have only used Geminin on human tissue.  It was from Proteintech Group cat# 10802-1-AP (1:1500 dilution using ER1 for 15 min-ER1=citrate pH6)

All of these were on FFPE tissues for brightfield only.  We did not use them for IF.

If you need any other specifics, let me know.

Ashley Troutman BS, MBA, HT(ASCP)QIHC
Supervisor-Translational Pathology Shared Resource
Vanderbilt University Medical Center
S-1310 Medical Center North
1161 21st Avenue South
Nashville, TN  37232


Message: 2

Date: Tue, 2 Dec 2014 19:37:51 +0000

From: "Swartwood, Steven J" <Steven.Swartwood <@t> cshs.org<mailto:Steven.Swartwood <@t> cshs.org>>

Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry

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Hello all,



I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects.



Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies.



I'd be grateful for any information from what clone/company do you buy from and  retrieval methods to primary antibody incubations/dilutions.



Thank you all for any information,



Steven Swartwood HT(ASCP)

Cedars Sinai Medical Center

steven.swartwood <@t> cshs.org<mailto:steven.swartwood <@t> cshs.org>