[Histonet] Re cd4 1F6 clone

[Neeraj Singh]

Try with no H2O2 at all for this clone. it will hill you.

Thanks

Neeraj


On Aug 30, 2014, at 1:40 PM, Steven Weston <Steven.Weston <@t> utas.edu.au> wrote:

> Hi Histonetters
> Has anyone else had difficulty getting  this clone to work? cd4 1F6 from leica microsystems (formerly novocastra).
> I've tried just about every trick in my book and still no staining.
> Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked.
> Anyone else with the same experience ?
> regards
> steve weston
> lab manager
> Breathe-Well CRE
> UTAS-SOM