[Histonet] ZDHHC11

[Swartwood, Steven J]

Hello Histonetters,

This is all pertaining to staining human tissues,

I work in a research core lab and was wondering if anyone had IHC-P (FFPE) experience in staining for ZDHHC11? From the data our computational team has pulled 70% of primary prostate ca is positive and 90% of metastatic prostate ca is positive. There's not much information out there for IHC-P staining with this antibody. The researcher that wants this staining done bought this from Thermo Scientific and they don't have much information either... other than a picture of lung adenocarcinoma that about 4 other companies also show from the same antibody. One company advised using pepsin digestion along with citrate retrieval and another said tris-EDTA. I figure to try out multiple retrievals (as usual with new antibodies), but has anyone ever used this antibody? I was trying to find some good control tissue as well (besides the cancer tissues) and all I can find is normal ovary so far. We have a Discovery Ultra here and I've tried a titer with a few different high grade primary prostate cancer tissues with different HIER and was unsuccessful, but I've yet to try the enzymatic approach.

Any help would be tremendously appreciated,

Steven Swartwood HT(ASCP)
Cedars Sinai Medical Center
steven.swartwood <@t> cshs.org

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