[Histonet] RE: Tissue falling off

[Susan.Walzer <@t> HCAHealthcare.com]

Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have  tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference.

Susan Walzer HT(ASCP)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, August 15, 2014 1:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue falling off

Hi - Just a couple of suggestions -
I've seen this periodically crop up during the years.  There are a couple of things to consider:
Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol.
Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all.  We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off.
Your water in the waterbaths is clean - Fresh DI water.  Once we had a contaminant in our DI water, and the patient tissues slid right off our slides.  We use clean slides, good quality water and super clean waterbaths.
Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers.  We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle.  We now send a rack through first, which turns on the heaters, then load the patient rack.
Hope this gives you a few ideas
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

   7. tissue falling off slides (Burnett, Brandy)   
From: "Burnett, Brandy" <bburnett <@t> CapeCodHealth.org>
Subject: [Histonet] tissue falling off slides

Our lab has encountered an issue with tissue falling off of the slides.
It is mostly the patient tissue
that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated.
Thanks Again,
Brandy Burnett, HTL


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