[Histonet] Bubbles in mounting media

[Jamal]

Hi Melissa
Try to fill the tube of mounting media some hours before use.
I use to fill it in the end of the day and in the next day the micro bubbles
will be completely disappeared.


Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowaihi <@t> alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Melissa Ng
Sent: Thursday, August 07, 2014 11:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bubbles in mounting media

Dear Histonet,

I am having a major problem with bubbles in my mounting media. I am
currently using the Leica coverslipper and Cellpath mounting media (Phalate
free). Micro-bubbles are observed on the slides after sometime of
coverslipping. In fact, when I checked the media, I found lots of bubbles
inside the bottle too! There were no bubbles in the bottle prior to placing
the mounting media with the machine (I checked!). Also, we do not see any
bubbles in the tubes that lead to the probe!

Previously, we were using the Leica mounting media (yellowish liquid) and
there were no bubble issues. I have checked with other labs using the
Cellpath mounting media (they use Leica coverslipper too) and no one seems
to be having the same problem as us!


The Cellpath mounting media is way more viscous compared to that of the
Leica media.

Has anyone here experienced a similar issue, and how have you resolved it?

Appreciate all the help I can get!

Best regards,
Melissa

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