[Histonet] RE: Decalcifying and Processing large bone
Huynh,Thomas
thomas.huynh <@t> mdanderson.org
Wed Aug 6 10:20:38 CDT 2014
Hi Bret
We are using 10% Formic Acid as well and checking the decalcifying end-point by using the faxitron x-ray system. I work with bone cancer for 99% of the time and Formic Acid is safer than any other one.
Thomas
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, August 06, 2014 10:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 129, Issue 6
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Today's Topics:
1. RE: Protocol for decalcifying and processing large bone
section. (DiCarlo, Margaret)
2. AW: [Histonet] RE: whole slide scanners (Gudrun Lang)
3. Glassware Cleaning, CAP Checklist Item GEN.41770 (Laurie Colbert)
4. RE: RE: whole slide scanners (Elizabeth Chlipala)
5. Listservers in microbiology (Jorge A. Santiago-Blay)
6. AW: [Histonet] RE: whole slide scanners (Gudrun Lang)
7. Procedures and Policy manual needed (Dr. Max Mirot)
8. RE: Glassware Cleaning, CAP Checklist Item GEN.41770
(Cooper, Brian)
9. RE: RE: whole slide scanners (Morken, Timothy)
10. RE: Listservers in microbiology (Cartun, Richard)
11. RE: RE: whole slide scanners (James Watson)
12. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770
(Joelle Weaver)
13. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Jamal)
14. Re: Protocol for decalcifying and processing large bone
section. (Barry Rittman)
15. Re: RE: Glassware Cleaning, CAP Checklist Item GEN.41770
(Michael Ann Jones)
16. RI company seeks plastics company for research work
(Hans B Snyder)
17. p16 (Sarah.Dysart <@t> stdavids.com)
18. neutralize formalin (Mary Ann Deathridge)
----------------------------------------------------------------------
Message: 1
Date: Tue, 5 Aug 2014 17:01:16 +0000
From: "DiCarlo, Margaret" <MDiCarlo <@t> KaleidaHealth.Org>
Subject: RE: [Histonet] Protocol for decalcifying and processing large
bone section.
To: "'Clough, Bret'" <Clough <@t> medicine.tamhsc.edu>, Histonet list serv.
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<731BE09CDB19AA43AA8682199D42D31B1DC79F59 <@t> ADCEXCHANGE01.KaleidaHealth.org>
Content-Type: text/plain; charset="us-ascii"
I use 10% formic acid for large human bones but I don't think your bone sections are that large.
Peggy DiCarlo
Ortho Bone Lab
BGMC
100 High St.
Buffalo, NY 14203
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clough, Bret
Sent: Tuesday, August 05, 2014 11:55
To: Histonet list serv.
Subject: [Histonet] Protocol for decalcifying and processing large bone section.
Hi everyone,
I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated!
Sincerely,
Bret Clough
Texas A&M Health Science Center
Temple, TX.
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Message: 2
Date: Tue, 5 Aug 2014 19:12:42 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] RE: whole slide scanners
To: "'Elizabeth Chlipala'" <liz <@t> premierlab.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
for which purpose do you use the high troughput scanner. Archive or
diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Elizabeth
Chlipala
Gesendet: Dienstag, 05. August 2014 17:16
An: James Watson; 'Kalleberg, Kristopher';
histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: whole slide scanners
I agree with James the type of scanner you need will be dependent upon your
use cases and workload. We have an Aperio ScanScope XT which is an 120
slide scanner and that works well for us.
I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo
and Jesus Ellin will be giving at the annual meeting in Austin this year,
it's called Digital Pathology for the Histotech - A Guide to Implementation
it's an all day workshop that is on Saturday the 23rd and it will cover all
aspects of how to select a scanner, how to implement scanning, etc. It will
be very valuable to histotechs that are already scanning and also techs that
are new to the scanning process.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Tuesday, August 05, 2014 8:14 AM
To: 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: whole slide scanners
The scanner you get depends on your usage and volume. Three that I
recommend are for high volume.
Aperio,
Good image quality, if you need to link to study metadata Spectrum software
is great. Recommend for chromogen. Separate scanner for fluorescence,
fluorescent scanner only holds 5 slides at a time.
Hamamatsu Nanozoomer,
What we use, we do high volume chromogen and fluorescence, excellent for
both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up
about 100 fluorescent slides per night, auto focus works well.
Philips,
Chromogen only, very fast scanning with high quality scans. 60 slides per
hour.
There are many others on the market for smaller workloads.
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation Scientific
Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915
jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg,
Kristopher
Sent: Tuesday, August 05, 2014 5:18 AM
To: histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole slide scanners
Hello All,
I am looking into the purchase of a whole slide scanner. If anyone could
supply some recommendations it would be greatly appreciated. Thank you.
Kris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 5 Aug 2014 18:05:49 +0000
From: Laurie Colbert <lcolbert <@t> pathmdlabs.com>
Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<12ECD7346266D74691EC2BFC75285E452F4B1C6D <@t> BFL323E10.pathmdlabs.local>
Content-Type: text/plain; charset="us-ascii"
Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
------------------------------
Message: 4
Date: Tue, 5 Aug 2014 12:21:00 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] RE: whole slide scanners
To: Gudrun Lang <gu.lang <@t> gmx.at>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE019C79ECD460 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="iso-8859-1"
We are a research based histology lab so we use scanning for several reasons:
1. We scan slides for clients so they can review their images remotely and capture snapshot images, annotate, etc- study sizes may range from 10 to 100's of slides
2. We scan all of our slides for IHC protocol development - our clients that request us to develop IHC staining protocols we scan slides so they can also view the slides and track our progress, plus this creates a great way for us to review the results too, we can easily determine the correct dilution by viewing multiple dilutions at the same time.
3. We scan all of our stained slides from the control blocks and these are linked to our control block database so anyone can view the scanned slide to see if the controls are working properly.
4. We have studies that require image analysis so all of the slides are scanned and then analyzed via a custom algorithm that will generate the data required for a particular study design.
5. We scan all of our IHC control slides so we can tract staining consistency
These are just a few of the applications that we use WSI for.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at]
Sent: Tuesday, August 05, 2014 11:13 AM
To: Elizabeth Chlipala
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: whole slide scanners
Hi,
for which purpose do you use the high troughput scanner. Archive or diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala
Gesendet: Dienstag, 05. August 2014 17:16
An: James Watson; 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: whole slide scanners
I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us.
I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Tuesday, August 05, 2014 8:14 AM
To: 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: whole slide scanners
The scanner you get depends on your usage and volume. Three that I recommend are for high volume.
Aperio,
Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time.
Hamamatsu Nanozoomer,
What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well.
Philips,
Chromogen only, very fast scanning with high quality scans. 60 slides per hour.
There are many others on the market for smaller workloads.
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Tuesday, August 05, 2014 5:18 AM
To: histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole slide scanners
Hello All,
I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you.
Kris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Tue, 5 Aug 2014 14:39:18 -0400
From: "Jorge A. Santiago-Blay" <blayjorge <@t> gmail.com>
Subject: [Histonet] Listservers in microbiology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAGBdDuDUoo32Hd3CBsEw_b3PZXVDh3f_XVpKGUqYq1mO45--kQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello:
I have found Histonet quite useful and the members with whom I have
communicated helpful. Can someone recommend listservers with similar
qualities that emphasize microbiology? If "yes", please feel free to email
at blayjorge <@t> gmail.com
Gratefully,
Jorge
Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html
------------------------------
Message: 6
Date: Tue, 5 Aug 2014 20:54:07 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] RE: whole slide scanners
To: "'Gudrun Lang'" <gu.lang <@t> gmx.at>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <000a01cfb0de$a31e6460$e95b2d20$@gmx.at>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone out there use digital pathology for routine diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Dienstag, 05. August 2014 19:13
An: 'Elizabeth Chlipala'
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: AW: [Histonet] RE: whole slide scanners
Hi,
for which purpose do you use the high troughput scanner. Archive or
diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Elizabeth
Chlipala
Gesendet: Dienstag, 05. August 2014 17:16
An: James Watson; 'Kalleberg, Kristopher';
histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: whole slide scanners
I agree with James the type of scanner you need will be dependent upon your
use cases and workload. We have an Aperio ScanScope XT which is an 120
slide scanner and that works well for us.
I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo
and Jesus Ellin will be giving at the annual meeting in Austin this year,
it's called Digital Pathology for the Histotech - A Guide to Implementation
it's an all day workshop that is on Saturday the 23rd and it will cover all
aspects of how to select a scanner, how to implement scanning, etc. It will
be very valuable to histotechs that are already scanning and also techs that
are new to the scanning process.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Tuesday, August 05, 2014 8:14 AM
To: 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: whole slide scanners
The scanner you get depends on your usage and volume. Three that I
recommend are for high volume.
Aperio,
Good image quality, if you need to link to study metadata Spectrum software
is great. Recommend for chromogen. Separate scanner for fluorescence,
fluorescent scanner only holds 5 slides at a time.
Hamamatsu Nanozoomer,
What we use, we do high volume chromogen and fluorescence, excellent for
both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up
about 100 fluorescent slides per night, auto focus works well.
Philips,
Chromogen only, very fast scanning with high quality scans. 60 slides per
hour.
There are many others on the market for smaller workloads.
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation Scientific
Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915
jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg,
Kristopher
Sent: Tuesday, August 05, 2014 5:18 AM
To: histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole slide scanners
Hello All,
I am looking into the purchase of a whole slide scanner. If anyone could
supply some recommendations it would be greatly appreciated. Thank you.
Kris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 5 Aug 2014 18:57:54 +0000
From: "Dr. Max Mirot" <Max.Mirot <@t> svrhc.org>
Subject: [Histonet] Procedures and Policy manual needed
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E7D668C2C687134B9FAE3268D55741AC023BD5 <@t> svexch05.svrhc.org>
Content-Type: text/plain; charset="us-ascii"
I am attempting to re-write our procedure and policy manual.
Our scope of service is routine anatomic pathology/histology and Non Gyn cytology. Right now the lab is CLIA certified.
Anyone care to share their policy and procedure manual?
I would like to look at a couple of nicely done manuals to serve as template. It can be for CAP not just CLIA.
Send off list to me max.mirot <@t> svrhc.org<mailto:max.mirot <@t> svrhc.org>
Thanks
Max S. Mirot, M.D.
------------------------------
Message: 8
Date: Tue, 5 Aug 2014 19:07:05 +0000
From: "Cooper, Brian" <bcooper <@t> chla.usc.edu>
Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item
GEN.41770
To: "Laurie Colbert" <lcolbert <@t> pathmdlabs.com>, "Histonet Post
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C12647AD4408834C8AB48FB0389C63E331402B4B <@t> CHLAEXMBH01.LA.AD.CHLA.ORG>
Content-Type: text/plain; charset=iso-8859-1
I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly.
Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357???? Pager: 213-209-0184
bcooper <@t> chla.usc.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, August 05, 2014 11:06 AM
To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 9
Date: Tue, 5 Aug 2014 19:07:50 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] RE: whole slide scanners
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF36790AED <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1
We are setting up Philips high speed slide scanners to scan frozen sections for remote Dx. One critical aspect is to get coverslip medium that dries fast...
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, August 05, 2014 10:13 AM
To: 'Elizabeth Chlipala'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: whole slide scanners
Hi,
for which purpose do you use the high troughput scanner. Archive or diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala
Gesendet: Dienstag, 05. August 2014 17:16
An: James Watson; 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: whole slide scanners
I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us.
I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Tuesday, August 05, 2014 8:14 AM
To: 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: whole slide scanners
The scanner you get depends on your usage and volume. Three that I recommend are for high volume.
Aperio,
Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time.
Hamamatsu Nanozoomer,
What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well.
Philips,
Chromogen only, very fast scanning with high quality scans. 60 slides per hour.
There are many others on the market for smaller workloads.
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Tuesday, August 05, 2014 5:18 AM
To: histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole slide scanners
Hello All,
I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you.
Kris
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_______________________________________________
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------------------------------
Message: 10
Date: Tue, 5 Aug 2014 19:15:46 +0000
From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
Subject: RE: [Histonet] Listservers in microbiology
To: "Jorge A. Santiago-Blay" <blayjorge <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9215BD4B0BA1B44D962A71C758B68D2E355C2F96 <@t> HHCEXCHMB03.hhcsystem.org>
Content-Type: text/plain; charset="utf-8"
Our Director of Microbiology gave me this information:
"Yes there is an excellent Listserver in microbiology but it is by invitation and requires ASM membership. Clinmicronetgroup."
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay
Sent: Tuesday, August 05, 2014 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Listservers in microbiology
Hello:
I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjorge <@t> gmail.com
Gratefully,
Jorge
Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
------------------------------
Message: 11
Date: Tue, 5 Aug 2014 19:30:32 +0000
From: James Watson <JWatson <@t> gnf.org>
Subject: RE: [Histonet] RE: whole slide scanners
To: 'Gudrun Lang' <gu.lang <@t> gmx.at>, 'Elizabeth Chlipala'
<liz <@t> premierlab.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A032B25305EC6949AC9AFF23C9620616C9F3B4EF <@t> EX5.lj.gnf.org>
Content-Type: text/plain; charset="iso-8859-1"
We are pharma research, about 40-45% of our slides are fluorescent. With 1 scanner.
2013 17,096 slides scanned
2013 20,501 slides scanned
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel??? 858-332-4647
Fax?? 858-812-1915
jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, August 05, 2014 10:13 AM
To: 'Elizabeth Chlipala'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: whole slide scanners
Hi,
for which purpose do you use the high troughput scanner. Archive or diagnostics?
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala
Gesendet: Dienstag, 05. August 2014 17:16
An: James Watson; 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: whole slide scanners
I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us.
I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Tuesday, August 05, 2014 8:14 AM
To: 'Kalleberg, Kristopher'; histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: whole slide scanners
The scanner you get depends on your usage and volume. Three that I recommend are for high volume.
Aperio,
Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time.
Hamamatsu Nanozoomer,
What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well.
Philips,
Chromogen only, very fast scanning with high quality scans. 60 slides per hour.
There are many others on the market for smaller workloads.
James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson <@t> gnf.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Tuesday, August 05, 2014 5:18 AM
To: histonet-request <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole slide scanners
Hello All,
I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you.
Kris
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_______________________________________________
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_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Tue, 5 Aug 2014 19:33:20 +0000
From: Joelle Weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item
GEN.41770
To: "Cooper, Brian" <bcooper <@t> chla.usc.edu>, Laurie Colbert
<lcolbert <@t> pathmdlabs.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT149-W34211A5685AAD1DF896006D8E30 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: bcooper <@t> chla.usc.edu
> To: lcolbert <@t> pathmdlabs.com; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 5 Aug 2014 19:07:05 +0000
> CC:
> Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770
>
> I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly.
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357 Pager: 213-209-0184
> bcooper <@t> chla.usc.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
> Sent: Tuesday, August 05, 2014 11:06 AM
> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
>
> Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned.
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA 90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------------------------------------------
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain confidential
> or legally privileged information. Any unauthorized review, use, disclosure
> or distribution is prohibited. If you are not the intended recipient, please
> contact the sender by reply e-mail and destroy all copies of this original message.
>
> ---------------------------------------------------------------------
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>
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------------------------------
Message: 13
Date: Wed, 6 Aug 2014 11:25:48 +0300
From: "Jamal" <j.rowaihi <@t> alborglaboratories.com>
Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item
GEN.41770
To: "'Joelle Weaver'" <joelleweaver <@t> hotmail.com>, "'Cooper, Brian'"
<bcooper <@t> chla.usc.edu>, "'Laurie Colbert'" <lcolbert <@t> pathmdlabs.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<01f901cfb150$0864d680$192e8380$@rowaihi <@t> alborglaboratories.com>
Content-Type: text/plain; charset="windows-1256"
I am also the same as Joelle & Brian, It never been questioned by CAP
inspectors but since it's in the common checklist we have to follow that?s
why we have a policy with simple procedure and record, the procedure almost
the same as Brian procedure but some time we use the digital PH meter.
Best Regards,
Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
Medical Laboratories |? Mobile +966 503629832|
j.rowaihi <@t> alborglaboratories.com
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA |
Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
www.alborglaboratories.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joelle
Weaver
Sent: Tuesday, August 05, 2014 10:33 PM
To: Cooper, Brian; Laurie Colbert; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770
Do the same as Brian. It is not that involved really, and its not worth
getting a deficiency over.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: bcooper <@t> chla.usc.edu
> To: lcolbert <@t> pathmdlabs.com; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 5 Aug 2014 19:07:05 +0000
> CC:
> Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770
>
> I've never been asked by a CAP auditor either, but our internal inspectors
used to inquire about this in a previous lab all the time. We use
Bromocresol Purple as an indicator here, and we have a simple log sheet
wherein our crew just tests one piece of cleaned glassware each day. They
write the type of glassware they test, and place a checkmark in a "yellow"
or "purple" column based upon what they see. If "purple," they rewash,
retest and document accordingly.
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357 Pager: 213-209-0184
> bcooper <@t> chla.usc.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
> Sent: Tuesday, August 05, 2014 11:06 AM
> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
>
> Do others in Histology follow these CAP Guidelines for cleaning glassware?
I'm not sure if this question actually applies to Histology. I have never
had an inspector ask me about it, but I'm wondering if anyone has been
questioned.
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA 90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
> The information contained in this transmission may contain privileged and
confidential information, including patient information protected by federal
and state privacy laws. It is intended only for the use of the person(s)
named above. If you are not the intended recipient, you are hereby notified
that any review, dissemination, distribution, or duplication of this
communication is strictly prohibited. If you are not the intended recipient,
please contact the sender by reply email and destroy all copies of the
original message.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------------------------------------------
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
confidential
> or legally privileged information. Any unauthorized review, use,
disclosure
> or distribution is prohibited. If you are not the intended recipient,
please
> contact the sender by reply e-mail and destroy all copies of this original
message.
>
> ---------------------------------------------------------------------
>
>
> _______________________________________________
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------------------------------
Message: 14
Date: Wed, 6 Aug 2014 07:02:10 -0500
From: Barry Rittman <barryrittman <@t> gmail.com>
Subject: Re: [Histonet] Protocol for decalcifying and processing large
bone section.
To: "Histonet list serv." <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CA+0tsF0Oqy9u3rVVuSiT5gJRKDdzkmTrVM7u-it4d8jnGq=-mQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
I would recommend using Kristensen's "sodium formate/formic acid" mixture.
This is more gentle than formic acid alone and almost as rapid.
Barry
On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret <
MDiCarlo <@t> kaleidahealth.org> wrote:
> I use 10% formic acid for large human bones but I don't think your bone
> sections are that large.
>
> Peggy DiCarlo
> Ortho Bone Lab
> BGMC
> 100 High St.
> Buffalo, NY 14203
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clough, Bret
> Sent: Tuesday, August 05, 2014 11:55
> To: Histonet list serv.
> Subject: [Histonet] Protocol for decalcifying and processing large bone
> section.
>
> Hi everyone,
> I was hoping someone on histonet would being willing to help me by either
> sharing their protocol or advising me on decalcifying and processing large
> bone sections. The bone sections are from the femural head of sheep
> measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying
> the samples in 0.5M EDTA which is taking along time to decalcify this being
> the 19th day. Any advise would be greatly appreciated!
>
>
> Sincerely,
> Bret Clough
> Texas A&M Health Science Center
> Temple, TX.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The Keeping You Informed section of Kaleida Health`s website features a
> wealth of information, stories and pictures about our valued workforce and
> the tremendous momentum our organization is experiencing. Check us out at:
> www.kaleidahealth.org/kyi
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
> or previous e-mail messages attached to it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, or a person responsible for
> delivering it to the intended recipient, you are hereby notified that any
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> recipient, please call Kaleida Health?????s Technology Assistance Center at (716)
> 859-7777.
>
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------------------------------
Message: 15
Date: Wed, 6 Aug 2014 13:12:58 +0000
From: Michael Ann Jones <mjones <@t> metropath.com>
Subject: Re: [Histonet] RE: Glassware Cleaning, CAP Checklist Item
GEN.41770
To: "Cooper, Brian" <bcooper <@t> chla.usc.edu>, Laurie Colbert
<lcolbert <@t> pathmdlabs.com>, "Histonet Post
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D0078712.2433%mjones <@t> metropath.com>
Content-Type: text/plain; charset="us-ascii"
Ditto with Brian Cooper.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com
On 8/5/14, 1:07 PM, "Cooper, Brian" <bcooper <@t> chla.usc.edu> wrote:
>I've never been asked by a CAP auditor either, but our internal
>inspectors used to inquire about this in a previous lab all the time. We
>use Bromocresol Purple as an indicator here, and we have a simple log
>sheet wherein our crew just tests one piece of cleaned glassware each
>day. They write the type of glassware they test, and place a checkmark
>in a "yellow" or "purple" column based upon what they see. If "purple,"
>they rewash, retest and document accordingly.
>
>Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
>Department of Pathology and Laboratory Medicine
>Children's Hospital Los Angeles
>4650 Sunset Blvd MS#43- Los Angeles, CA 90027
>Ph: 323.361.3357 Pager: 213-209-0184
>bcooper <@t> chla.usc.edu
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
>Colbert
>Sent: Tuesday, August 05, 2014 11:06 AM
>To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
>Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
>
>Do others in Histology follow these CAP Guidelines for cleaning
>glassware? I'm not sure if this question actually applies to Histology.
> I have never had an inspector ask me about it, but I'm wondering if
>anyone has been questioned.
>
>Laurie Colbert, HT (ASCP)
>Histology Supervisor
>PATH MD
>8158 Beverly Blvd.
>Los Angeles, CA 90048
>(323) 648-3214 direct
>(424) 245-7284 main lab
>
>The information contained in this transmission may contain privileged and
>confidential information, including patient information protected by
>federal and state privacy laws. It is intended only for the use of the
>person(s) named above. If you are not the intended recipient, you are
>hereby notified that any review, dissemination, distribution, or
>duplication of this communication is strictly prohibited. If you are not
>the intended recipient, please contact the sender by reply email and
>destroy all copies of the original message.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Message: 16
Date: Wed, 6 Aug 2014 09:50:03 -0400
From: Hans B Snyder <hans <@t> histologistics.com>
Subject: [Histonet] RI company seeks plastics company for research
work
To: "histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAAYBjcsn1tH1zTvDMSKQt74AGV7J3Nub-fJaxV=99JuLETAx_g <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello All,
I have a company who needs: "a source of basic services for
sectioning of GMA blocks containing biomaterial devices and subsequent
H&E staining. The devices are cut for embedding to about 8mm in
length, and contain adhesives, thermoplastic membranes, various
scaffolds, and cells. Our group would do the embedding and transfer
samples to you, and would be looking for about 25-50 samples/month."
I would like to give him references so that he can email and or call
places he chooses. Please email me and I will forward all
information.
Thank you
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com
------------------------------
Message: 17
Date: Wed, 6 Aug 2014 09:51:09 -0500
From: <Sarah.Dysart <@t> stdavids.com>
Subject: [Histonet] p16
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<34C22BB94729434598D767D3F4EB95E0CFA038E519 <@t> FWDCWPMSGCMS03.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist.
Thanks
Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas 78758
(512)901-1220
------------------------------
Message: 18
Date: Wed, 6 Aug 2014 08:06:42 -0700
From: "Mary Ann Deathridge" <madeathridge <@t> pastnashville.com>
Subject: [Histonet] neutralize formalin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <eccb9838e4bc4e718999081d3a1dabd4 <@t> pastnashville.com>
Content-Type: text/plain; charset="us-ascii"
We pour our waste xylene/alcohol into a drum and the formalin waste in a
seperate drum for a waste service company to remove. Problem is space to
house 55 gal drums and we have more xylene/alc waste than formalin. Does
anyone neutralize formalin and dispose of it down the drain? If so can you
recommend the method/product utilized.
Xylene recyler is not an option.
Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax:
615-298-4141
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End of Histonet Digest, Vol 129, Issue 6
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