[Histonet] Re: Negative Controls

[Campbell, Tasha M.]

As of now, my lab took that as you just needed one negative slide per
run.  And that negative slide is getting the negative mouse or rabbit
serum.  Is this correct?

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Terri
Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls

On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must have a negative tissue
control, which to quote CAP: "must  show no staining of tissues known to
lack the antigen"
Any of the following can serve as a negative tissue control:
1. Multi tissue blocks.  These can provide simultaneous positive and
negative tissue controls and are considered "best practice"...
The type of negative tissue control used (i.e. separate sections,
internal controls, or multitissue blocks) must be specified in the
laboratory manual."
Thus sayeth CAP, the almighty.  Please see ANP.22570
Our lab has defined our negative controls as a piece of Uterus as the
negative tissue in a multitissue block as a negative tissue control for
most of our antibodies, though for a few that might be too reactive in
uterus, we use a piece of skin.
I hope this helps.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 7
From: Beth Brinegar <bbrinegarhtl <@t> gmail.com>
Subject: [Histonet] Negative controls
Hello fellow histonetters,

What is are other labs doing to satisfy the ANP.22570 QC - Antibodies
"Appropriate negative controls are used."


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