[Histonet] RE: Retinal detachment

[Elizabeth Chlipala]

Debra

In order for the retina to remain attached you MUST fix in Davidsons, 10% NBF and 4% PFA are not acceptable fixatives they will cause retinal detachment.  You also need to trim the eye on one edge prior to processing or it's just going to shrink up like a raisin and look like hell.   We section a lot of eye samples and it can be a bit tricky.  We do not collect the samples - they are received in 70% alcohol we trim one edge and process on a shorter processing cycle (around 20 minutes a station, same day processing) with 3 changes of Propar instead of xylene.

Good Luck - eyes can be tricky!

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra Siena
Sent: Thursday, April 24, 2014 2:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retinal detachment

Hi All,

I have a friend that needs help to prevent retinal detachment in mouse eyes.  Her question is below.  Thanks for any help that you can provide.

"We have tried Davidson's fixative before, it is ok. What we are trying to do is eliminate the retinal detachment, wherever it comes from (either the harvesting technique, fixative, or processing). Will you ask how long do we fix mouse and rat eyes in each of the fixatives (alcoholic formalin, zinc formalin and Davidson's). After fixation, we transfer to 70% ethanol, and it starts out on the processor in 70% ethanol for about a 7 hour processing program. Get back to me as soon as you can.  Thank you very much.


Debbie Siena
800.442.3573 ext. 229 | www.statlab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rooster
Sent: Thursday, April 24, 2014 2:08 PM
To: Britton, Josette C
Cc: <histonet <@t> lists.utsouthwestern.edu>; Duddey, Aimee
Subject: Re: [Histonet] I need opinions on Leica instruments

Love them also!

Chris

On Apr 24, 2014, at 9:11 AM, "Britton, Josette C" <JCBRITTON <@t> Cheshire-Med.COM> wrote:

> I love all of their instruments! Josie Britton, HT (ASCP) QIHC
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Duddey, Aimee
> Sent: Thursday, April 24, 2014 7:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] I need opinions on Leica instruments
> 
> I am considering the Peloris II tissue processor and the Bond IHC 
> stainer for our lab.  We are a 400 bed regional hospital with a 
> growing outreach business.  We have had Thermo Excelsior processor for 
> years and have never really been happy with it.  Of course our VIP is 
> as reliable and consistent as they come.  We currently have a full 
> Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer).  I 
> would appreciate any feedback about Leica's line of products.  Please 
> include experiences good and bad.
> Thank you in advance.
> 
> Aimee M. Duddey, MLT(ASCP)
> Assistant Director of Laboratory - Pathology FirstHealth Moore 
> Regional Hospital Pinehurst, NC 28374
> (910) 715-5286
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet