[Histonet] Retinal detachment

[Debra Siena]

Hi All,

I have a friend that needs help to prevent retinal detachment in mouse eyes.  Her question is below.  Thanks for any help that you can provide.

"We have tried Davidson's fixative before, it is ok. What we are trying to do is eliminate the retinal detachment, wherever it comes from (either the harvesting technique, fixative, or processing). Will you ask how long do we fix mouse and rat eyes in each of the fixatives (alcoholic formalin, zinc formalin and Davidson's). After fixation, we transfer to 70% ethanol, and it starts out on the processor in 70% ethanol for about a 7 hour processing program. Get back to me as soon as you can.  Thank you very much.


Debbie Siena
800.442.3573 ext. 229 | www.statlab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rooster
Sent: Thursday, April 24, 2014 2:08 PM
To: Britton, Josette C
Cc: <histonet <@t> lists.utsouthwestern.edu>; Duddey, Aimee
Subject: Re: [Histonet] I need opinions on Leica instruments

Love them also!

Chris

On Apr 24, 2014, at 9:11 AM, "Britton, Josette C" <JCBRITTON <@t> Cheshire-Med.COM> wrote:

> I love all of their instruments! Josie Britton, HT (ASCP) QIHC
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Duddey, Aimee
> Sent: Thursday, April 24, 2014 7:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] I need opinions on Leica instruments
> 
> I am considering the Peloris II tissue processor and the Bond IHC 
> stainer for our lab.  We are a 400 bed regional hospital with a 
> growing outreach business.  We have had Thermo Excelsior processor for 
> years and have never really been happy with it.  Of course our VIP is 
> as reliable and consistent as they come.  We currently have a full 
> Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer).  I 
> would appreciate any feedback about Leica's line of products.  Please 
> include experiences good and bad.
> Thank you in advance.
> 
> Aimee M. Duddey, MLT(ASCP)
> Assistant Director of Laboratory - Pathology FirstHealth Moore 
> Regional Hospital Pinehurst, NC 28374
> (910) 715-5286
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