[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125,
Issue 24
Karen Kay
Karen.Kay <@t> albertahealthservices.ca
Mon Apr 21 12:27:10 CDT 2014
Good ;Morning,
We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment.
Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital - South Zone West - Alberta Health Services
Lethbridge, Alberta, Canada
karen.kay <@t> albertahealthservices.ca
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: April 21, 2014 11:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 125, Issue 24
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Today's Topics:
1. RE: Xylene substitute for processing GI biopsies
(Joe W. Walker, Jr.)
2. Alport's testing (Houston, Ronald)
3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa)
4. Recommendations cryostat (Pathology-Histology Sr. Supervisor)
5. RE: Paraffin (Cameron, Elizabeth)
6. COM 30450 (Piche, Jessica)
7. RE: Recommendations cryostat (WILLIAM DESALVO)
8. RE: Recommendations cryostat (Bea DeBrosse-Serra)
9. anti mouse iNOS (Anna Hughes)
10. Re: RE: Recommendations cryostat (Kim Tournear)
----------------------------------------------------------------------
Message: 1
Date: Sun, 20 Apr 2014 23:36:05 +0000
From: "Joe W. Walker, Jr." <joewalker <@t> rrmc.org>
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
To: Rene J Buesa <rjbuesa <@t> yahoo.com>, Lori Gemeinhardt
<loreli173 <@t> mac.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3C2378778400AD448ADA6FD6BDB7CCCC182A088D <@t> RRMBX03.rrmc.local>
Content-Type: text/plain; charset="iso-8859-1"
Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub?
We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol.
Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701
P: 802.747.1790 F: 802.747.6525
Email joewalker <@t> rrmc.org www.rrmc.org
Our Vision:
To be the Best Community Healthcare System in New England
Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
Ren? J.
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt <loreli173 <@t> mac.com> wrote:
Hi there!
I have spent a lot of time reading the archives, but still need some specific and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming.
It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated.
I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point.
Thanks, in advance, for your help!
Lori
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This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail.
Thank You
------------------------------
Message: 2
Date: Mon, 21 Apr 2014 13:12:12 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] Alport's testing
To: Histonet <histonet <@t> lists.utsouthwestern.edu>,
"ihcrg <@t> googlegroups.com" <ihcrg <@t> googlegroups.com>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE533419E737 <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
Does anyone test for Alport's (alpha 1, 3 & 5 chains of collagen IV) on paraffin sections?
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
"One person with passion is better than forty people merely interested."
~ E.M. Forster
------------------------------
Message: 3
Date: Mon, 21 Apr 2014 06:56:23 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
To: "Joe W. Walker, Jr." <joewalker <@t> rrmc.org>, Lori Gemeinhardt
<loreli173 <@t> mac.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1398088583.10371.YahooMailNeo <@t> web120405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Theoretically YES!
Ren? J.
On Sunday, April 20, 2014 7:36 PM, "Joe W. Walker, Jr." <joewalker <@t> rrmc.org> wrote:
Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub?
We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol.
Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701
P: 802.747.1790? F: 802.747.6525
Email joewalker <@t> rrmc.org? ? www.rrmc.org
Our Vision:
To be the Best Community Healthcare System in New England
Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
Ren? J.
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt <loreli173 <@t> mac.com> wrote:
Hi there!
I have spent a lot of time reading the archives, but still need some specific and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.? I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!? This is completely new (to me), and rather overwhelming.
It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated.
I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point.
Thanks, in advance, for your help!
Lori
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail.
Thank You
------------------------------
Message: 4
Date: Mon, 21 Apr 2014 14:01:36 +0000
From: "Pathology-Histology Sr. Supervisor" <histo <@t> skm.org.pk>
Subject: [Histonet] Recommendations cryostat
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "tahseen0009 <@t> gmail.com" <tahseen0009 <@t> gmail.com>
Message-ID:
<BBFD4ECF5CBFAE4E936663D9EB7AA8E620B65EE1 <@t> exchmbx.skm.org.pk>
Content-Type: text/plain; charset="us-ascii"
Hi All,
We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo.
Regards
Muhammad Tahseen
SKMCH&RC
------------------------------
Message: 5
Date: Mon, 21 Apr 2014 14:15:29 +0000
From: "Cameron, Elizabeth" <ecameron1 <@t> midcoasthealth.com>
Subject: [Histonet] RE: Paraffin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B5F1B1BBFD0AAF43A0A9F660F1B421E47B65BC <@t> mchex2010.mch.midcoasthealth.com>
Content-Type: text/plain; charset="us-ascii"
I am still trying to figure out what I need to do for validation for CAP. Do I need to revalidate IHC and/or special stains if I change the paraffin on the processor? The paraffin I have in mind is supposed to work faster than the one we currently use for infiltration, so we may need to adjust times.
Thanks.
________________________________________
From: Boyd, Debbie M [DKBoyd <@t> chs.net]
Sent: Wednesday, April 16, 2014 10:17 AM
To: Cameron, Elizabeth; histonet <@t> lists.utsouthwestern.edu
Subject: RE: Paraffin
We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth
Sent: Wednesday, April 16, 2014 9:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin
Hello histonetters,
I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated.
Thank you,
Liz
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------------------------------
Message: 6
Date: Mon, 21 Apr 2014 14:26:17 +0000
From: "Piche, Jessica" <jpiche <@t> wtbyhosp.org>
Subject: [Histonet] COM 30450
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<631955447A364B45B9458D2905635110AC107C84 <@t> WIN08-MBX-01.wtbyhosp.org>
Content-Type: text/plain; charset="us-ascii"
Hello Everyone,
Does COM 30450 apply to Diff Quik kits and reagents used in special stains (other stain kits)? Or does the lot to lot testing only apply to things such as IHC?
Thank you,
Jessica Piche, HT(ASCP)
CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital
------------------------------
Message: 7
Date: Mon, 21 Apr 2014 07:28:46 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> outlook.com>
Subject: RE: [Histonet] Recommendations cryostat
To: "Pathology-Histology Sr. Supervisor" <histo <@t> skm.org.pk>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Cc: "tahseen0009 <@t> gmail.com" <tahseen0009 <@t> gmail.com>
Message-ID: <COL129-W1F8DB5A4538FC0D4ACAC4825E0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Best recommendation I can provide is the Leica 1950 was the hands down choice of the employees performing frozen sections at multiple hospital sites in my system. From my perspective, the instruments are very reliable, easy to use, easy to maintain and pathologists approve of the the slide quality.
William DeSalvo, BS HTL(ASCP)
> From: histo <@t> skm.org.pk
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 21 Apr 2014 14:01:36 +0000
> CC: tahseen0009 <@t> gmail.com
> Subject: [Histonet] Recommendations cryostat
>
> Hi All,
> We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo.
> Regards
> Muhammad Tahseen
> SKMCH&RC
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 21 Apr 2014 14:35:33 +0000
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: [Histonet] RE: Recommendations cryostat
To: "'Pathology-Histology Sr. Supervisor'" <histo <@t> skm.org.pk>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "tahseen0009 <@t> gmail.com" <tahseen0009 <@t> gmail.com>
Message-ID:
<CB69CD3FBEB5524CA851930C971288FD324CBB33 <@t> Exch10MB02.isis.local>
Content-Type: text/plain; charset="us-ascii"
Leica!
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor
Sent: Monday, April 21, 2014 7:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: tahseen0009 <@t> gmail.com
Subject: [Histonet] Recommendations cryostat
Hi All,
We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo.
Regards
Muhammad Tahseen
SKMCH&RC
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Mon, 21 Apr 2014 14:55:13 +0000
From: Anna Hughes <anna.c.hughes <@t> gsk.com>
Subject: [Histonet] anti mouse iNOS
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<56B8D7633148A9419B559EBE8E9CF5900E2DCD8A <@t> 019-SN1MPN4-151.019D.MGD.MSFT.NET>
Content-Type: text/plain; charset="us-ascii"
Hi Everyone!
Anyone have a good antibody for an anti mouse iNOS antibody that they especially like?
Thanks!
Anna
Anna C. Hughes
anna.c.hughes <@t> gsk.com
------------------------------
Message: 10
Date: Mon, 21 Apr 2014 09:12:58 -0700
From: Kim Tournear <kim.tournear <@t> yahoo.com>
Subject: Re: [Histonet] RE: Recommendations cryostat
To: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "tahseen0009 <@t> gmail.com"
<tahseen0009 <@t> gmail.com>
Message-ID: <C7472623-5EAD-4757-B1B8-54A60701AAE1 <@t> yahoo.com>
Content-Type: text/plain; charset=utf-8
Definitely Leica
Sent from the iPhone of Kim Tournear ???????? ????
On Apr 21, 2014, at 7:35 AM, Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com> wrote:
> Leica!
>
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 2855 Gazelle Ct.
> Carlsbad, CA 92010
> 760-603-2371
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Pathology-Histology Sr. Supervisor
> Sent: Monday, April 21, 2014 7:02 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: tahseen0009 <@t> gmail.com
> Subject: [Histonet] Recommendations cryostat
>
> Hi All,
> We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo.
> Regards
> Muhammad Tahseen
> SKMCH&RC
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 125, Issue 24
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