[Histonet] Re: bunsen burner at the embedding center

Paula Sicurello patpxs <@t> gmail.com
Fri Sep 20 10:44:50 CDT 2013

I worked for the Navy Hospital in San Diego, way back in the day, and we
did an extraction with fuming HCl (straight, 100% HCl for folks who didn't
know what fuming meant).  Several people would get nosebleeds every time
they performed the extraction.  I finally got one of those fans that looks
like an airplane propellor in a cage and pried open the painted shut
windows.  I dared anyone to stop me, no one did!  1 was quite gutsy for a
22 year old.
The Petty Officers had me make 10N NaOH and forgot to tell me how hot it
got.  I almost dropped it when I picked it up.

Plus I had to swim to work, both ways in a rip tide-battleing sharks and
jelly fish, while sloshing around in an earthquake!  It is San Diego after

Happy Friday!

Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: 919.684.2091
HIPAA Privacy Notification: This message and any accompanying documents are
covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521,
and contain information intended for the specific individual (s) only. This
information is confidential. If you are not the intended recipient or an
agent responsible for delivering it to the intended recipient, you are
hereby notified that you have received this document in error and that any
review, dissemination, copying or the taking of any action based on the
contents of this information is strictly prohibited . If you have received
this communication in error, please notify us immediately by e-mail, and
delete the original message.

On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen <lowenjeff <@t> hotmail.com> wrote:

> yet here we are......how much of what they tell us is hyperbole?
> > Date: Fri, 20 Sep 2013 14:50:24 +0000
> > From: mucram11 <@t> comcast.net
> > To: rsrichmond <@t> gmail.com
> > Subject: Re: [Histonet] Re: bunsen burner at the embedding center
> > CC: histonet <@t> lists.utsouthwestern.edu
> >
> >
> >
> > I actually knew the person you are speaking of and it was his favorite
> trick.  We should be careful these younger people are using gloves for
> everything now to protect themselves.  W hen I started in Histology even
> the pathologists cleaned the paraffin off their hands with xylene and
> encouraged everyone to do the same .  We used so many things that are now
> not even allowed to be open on this planet and did know how dangerous or
> serious the possible issues could be over time.
> >
> >
> > Pam Marcum
> >
> >
> >
> > ----- Original Message -----
> > From: "Bob Richmond" <rsrichmond <@t> gmail.com>
> > To: "Histonet <@t> lists.utsouthwestern.edu" <
> histonet <@t> lists.utsouthwestern.edu>
> > Sent: Friday, September 20, 2013 9:35:55 AM
> > Subject: [Histonet] Re: bunsen burner at the embedding center
> >
> > I distinctly remember when I was a resident at Johns Hopkins in the late
> > 1960s that the histotechs would smoke while staining and coverslipping
> > without much ventilation. When I suggested to the chief technologist (who
> > later died of smoking related disease at 65, but at least he hadn't set
> > himself afire) that this wasn't such a good idea, he responded by
> stubbing
> > out a lighted cigarette in a Stender dish full of xylene (apparently you
> > can do this trick with gasoline also, but don't try it at home please).
> >
> > Buffering formalin was prohibited back then, and they removed the
> formalin
> > pigment by passing the sections through a concentrated solution (20 or
> 30%)
> > of picric acid in acetone. I'm glad he didn't try the cigarette trick in
> > THAT Stender dish.
> >
> > Fast-forward nearly half a century, and in the three labs I'm working in
> > I'm still grossing formalin-fixed tissue with minimal ventilation, but at
> > least people aren't allowed to smoke in the lab any more.
> >
> > Bob Richmond
> > Samurai Pathologist
> > Maryville TN
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list