[Histonet] ZInc formalin

Mesruh Turkekul turkekul <@t> gmail.com
Wed Sep 18 20:32:49 CDT 2013

Hi Liz,

I checked the web site and the images of FFPE sections stained with CD31 from Dianova. It seems that it mostly stains large blood vessels. Which I have experienced with most CD31 Ab on the market. Does this ab stain all the capillaries in your experiments?

Thanks again,

Sent from my iPad

On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:

> Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples.
> Liz
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz <@t> premierlab.com
> Ship to address:
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mesru T [turkekul <@t> gmail.com]
> Sent: Wednesday, September 18, 2013 4:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] ZInc formalin
> Dear Histonetters,
> I do not think there is a good CD31 (PECAM-1) antibody that will work on
> FFPE mouse tissues. If you have one please let me know. We use CD34 rat
> mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label
> endothelial cells of blood vessels in FFPE mouse tissues.
> There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose
> protected cryo embedded mouse tissues with antigen retrieval.
> The same CD31 Rat mono from BD will also work on Zinc Fixative  from BD
> cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but
> no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the
> section will be suboptimal due to this novel fixative.
> If anybody has any experince with this Zinc Fixative please would you share?
> Regards,
> Mesruh Turkekul
> mskcc.org
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