[Histonet] Re: Nail specimens

[Wanda Shotsberger AGray]

From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy
Sent: Tuesday, September 17, 2013 6:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nail specimens

Does anyone have a procedure for cutting nails and getting them to stay on 
the slide? Help please!

Thanks,
Cindy

Cindy,
We soak faced nail blocks in 1N sodium hydroxide for about 20 mins, then 
section @ 5um (pathologist preference), mount on charged slides and dry  @ 
65°C overnight. Cut beautifully and have very few wash offs, even after 
DPAS staining.
Good luck!
Wanda Shotsberger HT/HTL

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