[Histonet] Re: Nail specimens

Wanda Shotsberger AGray wgray19 <@t> sc.rr.com
Tue Sep 17 17:15:32 CDT 2013

From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy
Sent: Tuesday, September 17, 2013 6:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nail specimens

Does anyone have a procedure for cutting nails and getting them to stay on 
the slide? Help please!


We soak faced nail blocks in 1N sodium hydroxide for about 20 mins, then 
section @ 5um (pathologist preference), mount on charged slides and dry  @ 
65°C overnight. Cut beautifully and have very few wash offs, even after 
DPAS staining.
Good luck!
Wanda Shotsberger HT/HTL

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