[Histonet] Re: Histonet Digest, Vol 118, Issue 24
Anne
annigyg <@t> gmail.com
Fri Sep 13 00:46:55 CDT 2013
Choose the FILM coverslipper.
Sakura and DAKO over Leica anytime.
Annieinarabia
Sent from my iPhone
On 12 Sep 2013, at 9:02 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: Equipment selection (Huggins, Haley - MRMC)
> 2. Re: Equipment selection (Rene J Buesa)
> 3. RE: Equipment selection (Huggins, Haley - MRMC)
> 4. RE: Equipment selection (Marcum, Pamela A)
> 5. Need your expertise (Webb, Dorothy L)
> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 12 Sep 2013 08:55:39 -0700
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> Subject: RE: [Histonet] Equipment selection
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69 <@t> PHX-MSG-007-N2.chw.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future.
>
> Haley
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look?
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:Haley.Huggins <@t> DignityHealth.org>>
> To: "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Equipment selection
> To: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1379002076.53612.YahooMailNeo <@t> web163105.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper.
> René J.
>
>
> ________________________________
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
>
>
> I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future.
>
> Haley
>
> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look?
> RenéJ.
>
> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 3
> Date: Thu, 12 Sep 2013 09:10:47 -0700
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> Subject: RE: [Histonet] Equipment selection
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D <@t> PHX-MSG-007-N2.chw.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks, that is a good point.
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 9:08 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper.
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:Haley.Huggins <@t> DignityHealth.org>>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com<mailto:rjbuesa <@t> yahoo.com>>; "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
> I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future.
>
> Haley
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look?
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:Haley.Huggins <@t> DignityHealth.org>>
> To: "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 12 Sep 2013 16:30:13 +0000
> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
> Subject: RE: [Histonet] Equipment selection
> To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, "Huggins, Haley - MRMC"
> <Haley.Huggins <@t> DignityHealth.org>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57 <@t> Mail2Node2.ad.uams.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Actually the coverslipper from Leica is very good and can be linked to the stainer. It is also a glass coverslipper not tape. It is always about money and that may be the thing that decides no matter what any of prefers.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Thursday, September 12, 2013 11:08 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper.
> René J.
>
>
> ________________________________
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
>
>
> I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future.
>
> Haley
>
> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look?
> RenéJ.
>
> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ----------------------------------------------------------------------
> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 12 Sep 2013 11:45:41 -0500
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] Need your expertise
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823 <@t> HPEMX3.HealthPartners.int>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We will finally be going green by recycling formalin. I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge!
>
> Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's. We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers.
>
> As always, I do appreciate any and all comments, advice, or words of wisdom!! Hope to see some of you in Providence late next week!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Specialist
> 651-254-2962
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
>
> If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 12 Sep 2013 11:55:43 -0500
> From: Tim Higgins <thigginsht <@t> msn.com>
> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY179-W92030A0D22235B31AF8D97D83A0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hey Martha,
>
> You have to forgive Rene J. she speaks sometimes and has nothing good to say but keeps speaking. There is never a bad question, if you don't have a good answer then keep it to yourself as I usually do.
>
> "Adequate forum". really? I guess no one on Histonet is allowed to share their personal protocols, procedures, opinions, and experiences on here anymore.
>
> Come on Rene J., next time shhhhhhhhh!!
>
> I should have just hit the "Delete" button and follow my own advise!
>
> Tim N. Higgins, HT (ASCP), QIHC
>
>
>> Message: 7
>> Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT)
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] Minimum sample size for breast markers
>> To: Martha Ward-Pathology <mward <@t> wakehealth.edu>,
>> "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <1378992154.20733.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision.
>> Your pathologist is the one who should decide about this issue.
>> René J.
>>
>>
>> ________________________________
>> From: Martha Ward-Pathology <mward <@t> wakehealth.edu>
>> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Thursday, September 12, 2013 9:06 AM
>> Subject: [Histonet] Minimum sample size for breast markers
>>
>>
>>
>>
>> Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help.
>>
>> Martha Ward, MT (ASCP) QIHC
>> Manager
>>
>> Molecular Diagnostics Lab
>> Medical Center Boulevard \ Winston-Salem, NC 27157
>> p 336.716.2109 \ f 336.716.5890
>> mward <@t> wakehealth.edu
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 118, Issue 24
> *****************************************
More information about the Histonet
mailing list