[Histonet] Equipment selection
joelleweaver <@t> hotmail.com
Wed Sep 11 18:25:04 CDT 2013
I think all of these you listed are solid instruments. I am building a lab now, so recently had to pick instruments myself and would have liked to have had these options! From your list I would personally pick the Prisma and Bond Max , just on my personal experiences. I have a Leica ST5020 now, and while it is a good stainer, I hate the clips with the older model I have.
Consider if you will use glass or tape coverslipping, the multi-stainer glass coverslipper that goes with it is high maintenance, though it works fine when it works. I personally think the Prisma is great, has super throughput, nice staining. But used to only have tape coverslipping, but may have glass coverslips availible now-if that is a requirement of your pathologists. So check into that.
The need for a very open IHC platform ( considering if you do research v. clinical, use mostly concentrated v. prediluted antibodies, the training level of the staff, and your throughput needs as considerations; for example) may help in your choice. The DAKO is awesome , but check whether it still needs more off line pretreatment steps. This adds to the tech time, which might matter considering your staffing and volumes. The staining is fantastic if you can work with it and have staffing and your volumes are managable.
The Bond does all steps in automated runs, if you wish; I personally consider it middle ground in terms of "open-ness" somewhere between Ventana and Dako autostainer of years ago. The Bond is a super instrument in my opinion for the right lab situation. I use one now, and it works for me ( I do RTU and a fair number of concentrates) and a good number if ISH probes, and do all the tech work myself. I value the fast TAT with consistent staining-it does the job I need it to do.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: Haley.Huggins <@t> DignityHealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 11 Sep 2013 13:46:00 -0700
> Subject: [Histonet] Equipment selection
> Hi all,
> I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input.
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