[Histonet] esterase

[Stephen KumJew]

I was wondering if anyone knew of an esterase stain that doesn't stain the neuromuscular junction too dark. Have tried a modified technique by BJ Davis which uses naphthyl acetate, acetone 0.2M sodium phosphate and azotised pararosaniline but the staining was a dark red/black. Tried reducing times and temperature and reducing the pararosaniline but no change.

Thanks
Stephen

STEPHEN KUM JEW | Senior Technical Officer
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