[Histonet] RE: Grossing Prostate Biopsies

[Cesar Francisco Romero]

I gross
prostate needle biopsies every week.

I receive
12 samples but convert them in 6 paraffin blocks.

I stain
the Medial Samples of each level and each side with Eosin and the Lateral
Samples with EA - 36 .Example: Base Lateral Right ( EA - 36 ), with Base Medial Right ( Eosin ).Base Lateral Left ( EA - 36 ), with Base Medial Left ( Eosin ) and so on.

I wash
the excess of colorant with 96 alcohol, wrap the samples of each sextant  in one paper and put
them in one cassette.

Then I
put the Cassette in the First Alcohol to avoid washing the Stains with aqueous
formalin.

It is
very important to cut the cylinders so they do not exceed 5 mm. long.  You avoid the coiling and facilitate the
embedding.

After all
that it is very easy to see the colors in the paraffin block.It saves time and money and there is no errors if you are a tidy quiet person. Cesar RomeroBuenos AiresArgentina