[Histonet] cutting thick brain frozen sections for Golgi stain

[Candice Smoots]

Hi
 
I often section brain sections at 50 microns. However, I use a sliding microtome. For me, those are the best at sectioning thick brain sections. Vibratomes are great too. I would use the cryostat as a last resort. I also agree that if it is too cold, it wont section correctly and will begin to crack. Monitor the tem,p and make sure that it is not too cold.

I remain yours truely, 

Candice Camille 


________________________________
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
To: HISTONET <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, October 23, 2013 5:01 PM
Subject: [Histonet] cutting thick brain frozen sections for Golgi stain


I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415    Fax: 520.626.2097





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