[Histonet] RE: cutting thick brain frozen sections for Golgi stain

[Entwistle, Laura]

I routinely cut 50 or even 200um sections.  The brain should have some variations of light beige to it.  If it is too white, it is too cold.  What are you using?  I have found that a microtome works much better for thicker slices than a cryostat.

Good luck!
Laura

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kruttwig, Klaus
Sent: Wednesday, October 23, 2013 3:22 PM
To: Grantham, Andrea L - (algranth); HISTONET
Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain

Hi Andrea,

did you try it with a vibratome? If not, I would recommend it.

Thanks,
Klaus
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - (algranth) [algranth <@t> email.arizona.edu]
Sent: Wednesday, October 23, 2013 3:01 PM
To: HISTONET
Subject: [Histonet] cutting thick brain frozen sections for Golgi stain

I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





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