[Histonet] Re: cutting thick brain frozen section for Golgi stain

[Teri Johnson]

Hi Andi,

I second the option for Vibrotome section. I think that will be your best bet and should provide the least amount of artifact.

If you must use the cryostat, I agree that -18 is way too cold, go warmer than you are ordinarily comfortable with. If the OCT can't handle it, you might try using gelatin/sucrose as an embedding medium. Will you be staining free-floating sections?

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752