[Histonet] Cytopathology Quest.

Ian R Bernard ibernard <@t> uab.edu
Mon Oct 14 15:52:25 CDT 2013


Thanks for your reference and input
IB

-----Original Message-----
From: Fimbres, Amber [mailto:afimbres <@t> uci.edu] 
Sent: Monday, October 14, 2013 11:58 AM
To: Ian R Bernard; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Cytopathology Quest.

According to Koss' Diagnostic Cytology and its Histopathologic Bases (1979), 100% methanol is equivalent to 95% ethanol which is equivalent to 95% reagent (denatured) alcohol which is equivalent to 80% propanol which is equivalent to 80% isopropanol.  So in theory, if you ran out of 95% reagent alcohol and only had isopropanol on hand, as long as it was 80% it would be OK to use.  Have I tried this?  Honestly...no.

Amber Fimbres, CT(ASCP)HTL

-----Original Message-----
From: Ian R Bernard [mailto:ibernard <@t> uab.edu]
Sent: Sunday, October 13, 2013 6:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cytopathology Quest.

Per text books, for cytology specimens, 95% Ethyl Alcohol is the fixative of choice.  Are there any studies or references that approve 95% isopropyl or denatured alcohol as a suitable substitute?

Ian Bernard




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