[Histonet] Re: AFB Controls

[O'Donnell, Bill]

The only caution I would add to animal tissues is that they should be securely stored. In 1994, a group of animal rights activists opened the slide box and set all of my control slides free. 

Oh, yes, it's Friday.

Bill

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Thursday, October 10, 2013 9:37 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: AFB Controls

With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis.

I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis.

The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them.

Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys.

Bob Richmond
Samurai Pathologist
Maryville TN
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