[Histonet] Immunostaining after prolonged formalin immersion?

[Cartun, Richard]

I have obtained excellent immunoreactivity for some proteins on various tissues that have been in formalin for more than a year; however, you won't know unless you try it and there are "internal positive controls" to validate negative results.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Tim Wheelock [twheelock <@t> mclean.harvard.edu]
Sent: Wednesday, October 09, 2013 4:34 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Immunostaining after prolonged formalin immersion?

Hi Everyone:

If  tissue has been sitting in 10 % neutral buffered formalin for 3-5
years, can excellent immunostaining still be done on it.
I am guessing that some sort of antigen retrieval technique can be done
on the tissue, but I am not sure, since we don't do immunostaining
ourselves.

Thanks,

Tim

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA

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