[Histonet] Immunostaining after prolonged formalin immersion?

Tim Wheelock twheelock <@t> mclean.harvard.edu
Wed Oct 9 15:34:37 CDT 2013


Hi Everyone:

If  tissue has been sitting in 10 % neutral buffered formalin for 3-5 
years, can excellent immunostaining still be done on it.
I am guessing that some sort of antigen retrieval technique can be done 
on the tissue, but I am not sure, since we don't do immunostaining 
ourselves.

Thanks,

Tim

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA



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