[Histonet] Inconsistent Sections with Cryostat

[Perow, Elliott S (PEROWES10)]

Hello All,

I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another.  I am trying to section brook trout brain, but am having difficulty getting consistent sections.
I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block.  I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn.  I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine.  I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me.  Any help would be greatly appreciated.

Thanks,

Elliott Perow
Juniata College
Biology POE


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