[Histonet] RE: MOHS IPs
pruegg <@t> ihctech.net
pruegg <@t> ihctech.net
Wed Oct 2 09:35:33 CDT 2013
I agree with Barbara try fixing in cold acetone/ethanol mix even just for a couple of minutes then go directly to buffer do not dry
after what ever u u frozen sections i enzyme should help prevent ov incubating the primary? Are u usin detection? HRP/Dab or AEC or Alk.P/fast
-------- Original Message --------
Subject: [Histonet] RE: MOHS
From: Barbara Tibbs <[1]barbara.tibbs <@t> accuratediagnosticlabs.com>
D
To: "Bauer, Karen L." <[2]Bauer.Karen <@t> mayo.edu>,
"'histonet <@t> lists.utsouthwest
<[3]histonet <@t> lists.utsouthwestern.edu>
Hello Karen,
Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section
into a acetone in the cr minutes. Try diluting the Pr doesn't "eat" the tissue. This te doing ER/PR on frozen breast tumors. Not su but it's worth a try.
Barbara S. Tib
Histology Supervisor
Accurate Diagnostic Labs
South Pl
[4]barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
C
_____________________________________
From: [5]histonet-bounces <@t> lists.utsouthwestern.edu
<[6]histonet-bounces <@t> lists.utsouthwestern Karen L. <[7]Bauer.Karen <@t> mayo.edu>
Sent: Tuesday, October 01, 20
To: '[8]
Subject: [Histonet] MOHS IPs
Hi all,
We are in the process of validating some a lab. The Melan A (Mart 1) antibody is working well, darker. We will be increasing the Ab incubation time to se will help.
As for the Pan Keratin, we cannot get it Protease 2 on our Ultra stainer for FFPE tissues in this stain in MOHS is placed on fresh, unfixed tissue... by drop method. Any time we've tried to use an enzyme retrieval, th tissue looks eaten up... even if we incubate if for a minute.
would be retrieval.
For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your
protoc
Thanks so much!!
Karen
K MOHS Lab S [9]bauer.karen <@t> mayo.edu<[10]mailto:bauer.karen <@t> mayo.edu> | Mayo Clinic
Health System | 122 [11]mayoclinichealthsystem.org<http://www.[12]mayoclinichealthsystem.o
rg/>
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3. 3D"mailto:histonet <@t> lists.utsouthwestern.edu 4. 3D"mailto:barbara.tibbs <@t> accuratediagnosticlabs.c 5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu 6. 3D"mailto:histo 7. 3D"mailto:Bauer.Karen 8. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
9. 3D"mailto:bauer.kar 10. 3D"mailto:bauer.karen <@t> mayo 11. 3D"http://mayoclinichealt=/
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