[Histonet] RE: MOHS IPs

[pruegg <@t> ihctech.net]

   I  agree with Barbara try fixing in cold acetone/ethanol mix even    just  for  a  couple  of minutes then go directly to buffer do not dry
   after   what  ever  u  u   frozen  sections  i   enzyme  should  help  prevent  ov   incubating  the  primary?   Are  u usin   detection?  HRP/Dab or AEC or Alk.P/fast
   


     


   
-------- Original Message --------
   
Subject: [Histonet] RE: MOHS    
From: Barbara Tibbs <[1]barbara.tibbs <@t> accuratediagnosticlabs.com>
   
D   
To: "Bauer, Karen L." <[2]Bauer.Karen <@t> mayo.edu>,
   
"'histonet <@t> lists.utsouthwest   
<[3]histonet <@t> lists.utsouthwestern.edu>
   

   
Hello Karen,
      
Back  in the old days of doing IHC manually on mostly fresh, froze   n  tissue  I would immediately place the slide with the frozen section
   into  a   acetone  in  the  cr   minutes.  Try diluting the Pr   doesn't  "eat"  the  tissue.  This  te   doing ER/PR on frozen breast tumors. Not su   but it's worth a try.
   

   
Barbara S. Tib   
Histology Supervisor
   
Accurate Diagnostic Labs
   
South Pl   
[4]barbara.tibbs <@t> accuratediagnosticlabs.com
   
732-839-3374
   
C   

   

   
_____________________________________   
From:                  [5]histonet-bounces <@t> lists.utsouthwestern.edu
   <[6]histonet-bounces <@t> lists.utsouthwestern   Karen L. <[7]Bauer.Karen <@t> mayo.edu>
   
Sent: Tuesday, October 01, 20   
To: '[8]   
Subject: [Histonet] MOHS IPs

   
Hi all,
   

   
We  are  in the process of validating some a   lab.  The Melan A (Mart 1) antibody is working well,    darker.  We will be increasing the Ab incubation time to se   will help.
   

   
As  for  the Pan Keratin, we cannot get it    Protease  2  on  our Ultra stainer for FFPE tissues in   this  stain in MOHS is placed on fresh, unfixed tissue... by   drop  method.  Any  time we've tried to use an enzyme retrieval, th   tissue looks eaten up... even if we incubate if for a minute.
   

      would  be   retrieval.
      
For  those of you in MOHS labs that are using a manual staining me   thod  for  Melan A and Pan Keratin, would you be willing to share your
   protoc   

   
Thanks so much!!
   

   
Karen
   

   
K   MOHS   Lab   S   [9]bauer.karen <@t> mayo.edu<[10]mailto:bauer.karen <@t> mayo.edu> | Mayo Clinic
   Health  System  |  122   [11]mayoclinichealthsystem.org<http://www.[12]mayoclinichealthsystem.o
   rg/>
   

      
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References

   1. 3D"mailto:barbara.tibbs <@t> accurated   2. ="mailto:Bauer.Karen <@t> mayo.edu"
   3. 3D"mailto:histonet <@t> lists.utsouthwestern.edu   4. 3D"mailto:barbara.tibbs <@t> accuratediagnosticlabs.c   5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu   6. 3D"mailto:histo   7. 3D"mailto:Bauer.Karen   8. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   9. 3D"mailto:bauer.kar  10. 3D"mailto:bauer.karen <@t> mayo  11. 3D"http://mayoclinichealt=/
  12. 3D"http:/  13. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  14. 3D"http://lists.utsouthweste=/
  15. 3D"mailto:Histonet <@t> lists.u  16. file://localhost/tmp/3D"h