[Histonet] RE: Rush Processing Protocol

[Glenn Hauck]

I am most interested in a rush processes as well, we also only do overnight processing. We have a large variety of both large and small tissue types.

The reagents we have on board on our processors are listed below.
10 % NBFx1
70% ALCx1
80%ALCx2
95%ALCx2
100%ALCx2
Xylenex2
Paraffin- X 4

Glenn Hauck

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Friday, November 29, 2013 8:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH
Subject: [Histonet] Rush Processing Protocol

Fellow Histonetters, our laboratory mostly processes small biopsy specimens such as GI, skins, vas deferens, ECCs, Cervical biopsies, EMBXs and  breast core bxs with our largest routine specimen being gall bladders.

We are seeking recommendations on a proven same-day or less than 8-hour processing program that would facilitate processing rush specimens only. Of course, we would validate this protocol with our processor and reagents. Any suggestions? Looking for total time and optimum time in each reagent.  Our routine overnight is  little over a 12 hour protocol.

Please provide an evidence-based reference for optimum minimum processing time of biopsy tissues versus maximum time. Below are the reagents and amount of reagent containers we use.

10 % NBF- x 2
70% ALC- x 1
95% ALC- x 1
100% ALC- x 4
Clearing- X 2
Paraffin- X 4


Ian Bernard



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you.