[Histonet] RE: Rush Processing Protocol

Glenn Hauck Glenn.Hauck <@t> albertahealthservices.ca
Fri Nov 29 09:58:24 CST 2013

I am most interested in a rush processes as well, we also only do overnight processing. We have a large variety of both large and small tissue types.

The reagents we have on board on our processors are listed below.
10 % NBFx1
70% ALCx1
Paraffin- X 4

Glenn Hauck

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Friday, November 29, 2013 8:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rush Processing Protocol

Fellow Histonetters, our laboratory mostly processes small biopsy specimens such as GI, skins, vas deferens, ECCs, Cervical biopsies, EMBXs and  breast core bxs with our largest routine specimen being gall bladders.

We are seeking recommendations on a proven same-day or less than 8-hour processing program that would facilitate processing rush specimens only. Of course, we would validate this protocol with our processor and reagents. Any suggestions? Looking for total time and optimum time in each reagent.  Our routine overnight is  little over a 12 hour protocol.

Please provide an evidence-based reference for optimum minimum processing time of biopsy tissues versus maximum time. Below are the reagents and amount of reagent containers we use.

10 % NBF- x 2
70% ALC- x 1
95% ALC- x 1
100% ALC- x 4
Clearing- X 2
Paraffin- X 4

Ian Bernard

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