[Histonet] Over fixation

Tony Reilly Tony.Reilly <@t> health.qld.gov.au
Sun Nov 24 17:37:49 CST 2013

Hi Sanjeet

It is true that tissue can bleach over time in formalin making staining difficult.  This possibly happens if the formalin becomes acidic over time.  This can be improved by treating the tissue with 0.05% periodic acid followed by a water wash and then staining as usual.



Tony Reilly  B.App.Sc. , M.Sc.

Chief Scientist, Anatomical Pathology

Pathology Queensland-PA Laboratory 

Health Services Support Agency | Department of Health


Level 1, Building 15,Princess Alexandra Hospital

Ipswich Road,WOOLLOONGABBA  Qld 4102
Ph: 07 3176 2412
Mob: 0402 139411

Fax: 07 3176 2930

Email: tony.reilly <@t> health.qld.gov.au

Web:  www.health.qld.gov.au/qhcss/


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanjeet
Sent: Monday, 25 November 2013 6:33 AM
To: Tony Reilly; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Over fixation

Hi Histo folks,
Tissue biopsy that are  not fixed properly do have an adverse effect on H & E staining. Tissues can be kept in formalin for a period of 5 to 7 days as per literature. But what happens to the tissues that are kept over for a longer period of time. By that I mean 6 to 12 months. Would over fixed tissues have any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find that the over fixed tissues lose their affinity towards the dyes . Please advice. 

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