[Histonet] Has anyone had any problems with the Vector Immuedge pap pen?

[Kruttwig, Klaus]

Hi Patrick,

yes, I had a similar problem with the Immedge Pap pen. I took very long before I could immerse the sections again with buffer and the barrier still collapsed later on. I used a quite old one that I found in the lab so I guess they stop functioning after a while. 

Klaus

Klaus Kruttwig
Department of Cell and Tissue Biology 
University of California, San Francisco
HSW601
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Lewis, Patrick [patrick.lewis <@t> seattlechildrens.org]
Sent: Thursday, November 14, 2013 10:19 AM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Has anyone had any problems with the Vector Immuedge pap    pen?

Hi everyone,

Has anyone had any problems with the Immedge Pap pen from Vector. Cat # H-4000

It gives a nice wide barrier, but for some reason the barrier is disintegrating on our slides so I get holes in the lines I draw.

I have had a similar problem before, usually because the slide wasn't dry enough when I was drawing the barrier or I was immersing the slide before the barrier had a chance to solidify.   However, the problem has never been this bad.  I am wondering if my current batch of pap pens is defective.

Patrick





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