[Histonet] Has anyone had any problems with the Vector
Immuedge pap pen?
Klaus.Kruttwig <@t> ucsf.edu
Thu Nov 14 14:35:43 CST 2013
yes, I had a similar problem with the Immedge Pap pen. I took very long before I could immerse the sections again with buffer and the barrier still collapsed later on. I used a quite old one that I found in the lab so I guess they stop functioning after a while.
Department of Cell and Tissue Biology
University of California, San Francisco
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Lewis, Patrick [patrick.lewis <@t> seattlechildrens.org]
Sent: Thursday, November 14, 2013 10:19 AM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Has anyone had any problems with the Vector Immuedge pap pen?
Has anyone had any problems with the Immedge Pap pen from Vector. Cat # H-4000
It gives a nice wide barrier, but for some reason the barrier is disintegrating on our slides so I get holes in the lines I draw.
I have had a similar problem before, usually because the slide wasn't dry enough when I was drawing the barrier or I was immersing the slide before the barrier had a chance to solidify. However, the problem has never been this bad. I am wondering if my current batch of pap pens is defective.
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet