[Histonet] neuropath stains

Lewis, Sarah Sarah.Lewis <@t> nationwidechildrens.org
Thu Nov 14 11:04:16 CST 2013

Our immunofluorescence protein antibody stains are automated, but not the routine histochemical stains used for muscle biopsies.  Between the importance of maintaining pH levels, incubation temperatures (SDH,NADH,COX, AtPase's, Amyloid, MAD, Myophos, Acid Phos) and the need of a rotator plate (ORO).  Really the only options for automation would be the H&E, Gomori Trichrome  and PAS. 

Sarah E. Lewis HT, ASCP
Laboratory Coordinator
The Research Institute at
Nationwide Children's Hospital
Center for Gene Therapy
Neuromuscular Division
Rm WA3110

-----Original Message-----
From: Nails, Felton [mailto:flnails <@t> texaschildrens.org] 
Sent: Wednesday, November 13, 2013 5:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] neuropath stains

Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains?

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