[Histonet] unsubscribe

McCarty, Jean [JRDUS] JMcCarty <@t> its.jnj.com
Wed Nov 6 12:05:01 CST 2013



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, November 06, 2013 1:02 PM
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Subject: Histonet Digest, Vol 120, Issue 7

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Today's Topics:

   1. FW: Fungus Controls (Tim Higgins)
   2. RE: Histonet Digest, Vol 120, Issue 6 (Gray, Ed)
   3. RE: FDA Disclaimer (Hannen, Valerie) (Jean Wood)
   4. RE: FW: Fungus Controls (Tom McNemar)
   5. CD 34 antibody abcam (ab963) (tahseen)
   6. Water rinse for H&E's (Pardue, Judith)
   7. Re: Water rinse for H&E's (Rene J Buesa)
   8. RE: Water rinse for H&E's (Terri Brown)
   9. Biopsy Problems (Joanne Clark)


----------------------------------------------------------------------

Message: 1
Date: Tue, 5 Nov 2013 13:03:54 -0600
From: Tim Higgins <thigginsht <@t> msn.com>
Subject: [Histonet] FW: Fungus Controls
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	"Vickroy.Jim <@t> mhsil.com"
	<vickroy.jim <@t> mhsil.com>
Message-ID: <BAY179-W76F945A26CD401D94A23FED8F10 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"




I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains.  Its "naturally occurring", a petri dish would not be naturally occurring in my mind.
 
Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it.  Actually happy I was able to save some money.
 
Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!!  I have never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC
 

> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie <foreightl <@t> gmail.com>
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<CAKEyg-2KaDkn0oFQURzuPcugQt=Ju8LKK6C8daH+nPjvVsPMJw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi Jim,
> 
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
> 
> Good luck!
> 
> 
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:
> 
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
 		 	   		  

------------------------------

Message: 2
Date: Tue, 5 Nov 2013 19:20:42 +0000
From: "Gray, Ed" <egray <@t> hsc.wvu.edu>
Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<6a889df9e216449bbac73ab5d795aa71 <@t> BN1PR05MB406.namprd05.prod.outlook.com>
	
Content-Type: text/plain; charset="us-ascii"

You actually need the performing lab's disclaimer.  We've sent stains and other procedures to several other labs.  We refer to the disclaimers as ASR's (analyte specific reagents) and build templates for each lab's specific comments in our APLIS.

Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray <@t> wvuhealthcare.com

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Message: 9
Date: Tue, 5 Nov 2013 05:47:53 -0500
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] FDA Disclaimer
To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696 <@t> isexstore03>
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Good Morning...

I have a question about the FDA disclaimer for Immuno's.  If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer?

Thanks so much!!


Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com


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------------------------------

Message: 3
Date: Tue, 5 Nov 2013 13:41:14 -0800
From: Jean Wood <jwood <@t> fairchildmed.org>
Subject: [Histonet] RE: FDA Disclaimer (Hannen, Valerie)
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A4FD115D6 <@t> cliff>
Content-Type: text/plain; charset="us-ascii"

  Valerie,

 If your pathologist is reading your IHC send-outs then he is most likely contracted through that third party company (he gets PC compensation) and they do the technicial work. In our case, our pathologist signs out our routine H&E Final Surgical Pathology Report and when he reads the IHC  he uses their report portal to issue those results using their letterhead with the FDA disclaimer attached at the end of the report. Sometimes he may incorporate the IHC results in the original report as revised  but we always piggy back the third party IHC report (with digital pathology images) to the original.

It might be best to contact the IHC company representative and find out excatly who is legally responsible for the disclaimer - the technical service provider or the proffesional.

Jean Wood BS, HT
Fairchild Medical Center Pathology Dept.
Ph:530.841.6243 Fax:530.841.6232
jwood <@t> fairchildmed.org
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Tuesday, November 05, 2013 10:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 120, Issue 6

Send Histonet mailing list submissions to
        histonet <@t> lists.utsouthwestern.edu

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Today's Topics:

   1. Re: basement membranes (Lee & Peggy Wenk)
   2. (no subject) (Davis, Cassie)
   3. GMS fungus controls (Davis, Cassie)
   4. Re: Aspergillus tissue blocks for controls (Patrick Laurie)
   5. Problems with H&E Bleeding (P.E. Visser)
   6. AUTO: Bruce Palmatier is out of the office (returning
      11/08/2013) (Bruce_Palmatier <@t> vwr.com)
   7. Re: Aspergillus tissue blocks for controls (Hans B Snyder)
   8. RE: Aspergillus tissue blocks for controls (Diana McCaig)
   9. FDA Disclaimer (Hannen, Valerie)
  10. FW: The results of your email commands (Scott, Allison D)
  11. Hot Histology Career Opportunities Alert from Pam Barker  at
      RELIA Solutions!! Even if you are happy where you are take        a look
      please we offer a 500.00 referral fee and it's almost     time for
      Christmas shopping!!! 11/5/2013 (Pam Barker)
  12. (no subject) (Richard Yeo)
  13. Faxitron Xray digital imaging (Scott, Allison D)
  14. RE: Faxitron Xray digital imaging (WILLIAM DESALVO)


----------------------------------------------------------------------

Message: 1
Date: Mon, 4 Nov 2013 13:38:25 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] basement membranes
To: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>,
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3BC14F9010C4499A88E5351B42787F0B <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
        reply-type=original

Electron Microscope.

Even if you did a periodic acid-methenamine silver stain (PASM, Jones),
which in my opinion is the best histology stain, since it is a silver stain,
you can get different thicknesses of basement membrane (bm) by leaving it in
longer or shorter than is optimal for that patient's bm thickness.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message-----
From: Edwards, Richard E.
Sent: Monday, November 04, 2013 9:39 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] basement membranes


Best  technique, tinctorial or otherwise of identifying, with a  view to
measuring their width, many  thanks.


 Richard  Edwards


          Leicester University


                     U.K.
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 2
Date: Mon, 4 Nov 2013 13:45:26 -0500
From: "Davis, Cassie" <CDavis <@t> che-east.org>
Subject: [Histonet] (no subject)
To: "Vickroy.Jim <@t> mhsil.com" <Vickroy.Jim <@t> mhsil.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <08861B9CF6C7774E874635A4818AE37B01C9AC44F5 <@t> CHEXCMS01.one.ads.che.org>
Content-Type: text/plain; charset="iso-8859-1"

Jim,

We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095




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------------------------------

Message: 3
Date: Mon, 4 Nov 2013 13:46:23 -0500
From: "Davis, Cassie" <CDavis <@t> che-east.org>
Subject: [Histonet] GMS fungus controls
To: "Vickroy.Jim <@t> mhsil.com" <Vickroy.Jim <@t> mhsil.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
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        <08861B9CF6C7774E874635A4818AE37B01C9AC44F9 <@t> CHEXCMS01.one.ads.che.org>
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Jim,

We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095




Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
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------------------------------

Message: 4
Date: Mon, 4 Nov 2013 15:07:06 -0500
From: Patrick Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] Aspergillus tissue blocks for controls
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
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        <CAKEyg-2KaDkn0oFQURzuPcugQt=Ju8LKK6C8daH+nPjvVsPMJw <@t> mail.gmail.com>
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Hi Jim,

I have found a couple of ways.  First, if you are fortunate to have a micro
department nearby, they can make a very nice one for you (a Histotip in
Sakura's Histologic).  Another method, which doesn't work the best,  can be
moldy orange peel.  And the easiest, you can create your own (moldy
sausage) or even take some human tissue (fresh lung works the best) and
leave it with some moldy meat, then you can get a great one.

Good luck!


On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:

> We are in desperate needs of obtaining GMS controls.   In the old days we
> had all the fungal specimens we needed.  Today it is getting hard to find
> these tissues or controls.   Does anyone have any idea where we can get
> tissue blocks with Aspergillus or does anyone have any other suggestions
> about GMS controls.
>
> James Vickroy BS, HT(ASCP)
>
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
> ________________________________
> This message (including any attachments) contains confidential information
> intended for a specific individual and purpose, and is protected by law. If
> you are not the intended recipient, you should delete this message. Any
> disclosure, copying, or distribution of this message, or the taking of any
> action based on it, is strictly prohibited.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869


------------------------------

Message: 5
Date: Mon, 4 Nov 2013 21:28:33 +0100
From: "P.E. Visser" <pev <@t> xs4all.nl>
Subject: [Histonet] Problems with H&E Bleeding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002301ced99c$7098c9f0$51ca5dd0$@xs4all.nl>
Content-Type: text/plain;       charset="us-ascii"

Dear anna

I had while working for Baker chemicals, a customer in Germany who had a
similar problem there H&E turned purple. the red disappeared. the
environment around the section contained little red droplets (only visible
under high magnification). after some time (because at first they clamed
noting was changed) it became clear  that they skipped ethanol 100 % and
just used the cheaper 96% instead. when evaluated they tested 3 slides and
that worked. but after 300 - 500 slides the xylene was saturated with water.
eosin being soluble in water migrates from the section to the water emulate
in the xylene.

so I would test the ethanol you use. 3 times 100% is in my view a must.



I also on time in Scotland was confronted with xylene that was sold as non
recycled, but in fact it was. and sadly the supplier used an open container
to speed up cooling down. the rain was the source of the water. it lead to
several problems and took a long time before the we could find the source of
these problems.



wish you success with solving yours.

regards piet



------------------------------

Message: 6
Date: Mon, 4 Nov 2013 16:00:59 -0500
From: Bruce_Palmatier <@t> vwr.com
Subject: [Histonet] AUTO: Bruce Palmatier is out of the office
        (returning      11/08/2013)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <OF40219727.57C2C3FB-ON85257C19.0073729C-85257C19.0073729C <@t> vwr.com>
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I am out of the office until 11/08/2013.

I will be out of the office from Nov 4- Nov 8. If you have a question about
an order or need assistance placing an order, or if the matter is urgent,
please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service
associate will assist you. For other matters, I will respond to emails
within 24 hours. If you require pricing information, quotes, product
information, or other assistance, Tim Rafferty is the new VWR Healthcare
Sales Rep who's taken my place. He can be reached at
timothy_rafferty <@t> vwr.com or (717) 668-9045.

Thank You,

Bruce Palmatier
Market Portfolio Manager
VWR Healthcare
bruce_palmatier <@t> vwr.com
mobile: 484.319.5563
fax: 484-881-7307

Customer Service:  877.881.1192
Fax: 484.881.6486
Customer Service email: HEALTHCARESERVICE <@t> VWR.COM


Note: This is an automated response to your message  "Histonet Digest, Vol
120, Issue 5" sent on 11/4/2013 1:06:11 PM.

This is the only notification you will receive while this person is away.




------------------------------

Message: 7
Date: Mon, 4 Nov 2013 16:46:37 -0500
From: Hans B Snyder <hans <@t> histologistics.com>
Subject: Re: [Histonet] Aspergillus tissue blocks for controls
To: Patrick Laurie <foreightl <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    "Vickroy, Jim"
        <Vickroy.Jim <@t> mhsil.com>
Message-ID:
        <CAAYBjcsbZDbzLuD0OnWe4sA2mOvqU3KHNaon1vv49SFUWo0b=g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

We have tried the moldy peel also but the pathologists did not like this
and would not accept any controls of this form.  I don't blame them they
want to see it naturally occurring.

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com <haqns <@t> histologistics.com>


On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie <foreightl <@t> gmail.com> wrote:

> Hi Jim,
>
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
>
> Good luck!
>
>
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com>
> wrote:
>
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
> > ________________________________
> > This message (including any attachments) contains confidential
> information
> > intended for a specific individual and purpose, and is protected by law.
> If
> > you are not the intended recipient, you should delete this message. Any
> > disclosure, copying, or distribution of this message, or the taking of
> any
> > action based on it, is strictly prohibited.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
>
> Patrick Laurie(HT)ASCP QIHC
>
> Histology Manager
>
> Celligent Diagnostics, LLC
>
> 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262
>
> Work: 704-970-3300  Cell: 704-266-0869
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 8
Date: Mon, 4 Nov 2013 22:31:18 +0000
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
Subject: RE: [Histonet] Aspergillus tissue blocks for controls
To: "'Hans B Snyder'" <hans <@t> histologistics.com>, Patrick Laurie
        <foreightl <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    "Vickroy, Jim"
        <Vickroy.Jim <@t> mhsil.com>
Message-ID:
        <D8866AEBD70A70408259C98EB75DFA7209C9BA <@t> EXMBX1.esclhin.on.ca>
Content-Type: text/plain; charset="us-ascii"

As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements.  But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results.

Diana
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: November-04-13 4:47 PM
To: Patrick Laurie
Cc: histonet <@t> lists.utsouthwestern.edu; Vickroy, Jim
Subject: Re: [Histonet] Aspergillus tissue blocks for controls

We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form.  I don't blame them they want to see it naturally occurring.

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com <haqns <@t> histologistics.com>


On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie <foreightl <@t> gmail.com> wrote:

> Hi Jim,
>
> I have found a couple of ways.  First, if you are fortunate to have a
> micro department nearby, they can make a very nice one for you (a
> Histotip in Sakura's Histologic).  Another method, which doesn't work
> the best,  can be moldy orange peel.  And the easiest, you can create
> your own (moldy
> sausage) or even take some human tissue (fresh lung works the best)
> and leave it with some moldy meat, then you can get a great one.
>
> Good luck!
>
>
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com>
> wrote:
>
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other
> > suggestions about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor Memorial
> > Medical Center
> > 217-788-4046
> >
> >
> > ________________________________
> > This message (including any attachments) contains confidential
> information
> > intended for a specific individual and purpose, and is protected by law.
> If
> > you are not the intended recipient, you should delete this message.
> > Any disclosure, copying, or distribution of this message, or the
> > taking of
> any
> > action based on it, is strictly prohibited.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
>
> Patrick Laurie(HT)ASCP QIHC
>
> Histology Manager
>
> Celligent Diagnostics, LLC
>
> 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262
>
> Work: 704-970-3300  Cell: 704-266-0869
> _______________________________________________
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------------------------------

Message: 9
Date: Tue, 5 Nov 2013 05:47:53 -0500
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] FDA Disclaimer
To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu)"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696 <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"

Good Morning...

I have a question about the FDA disclaimer for Immuno's.  If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer?

Thanks so much!!


Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com


===================
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hereby notified that any dissemination, distribution, or
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delete this message. Thank you"
===================

------------------------------

Message: 10
Date: Tue, 5 Nov 2013 14:50:15 +0000
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
Subject: [Histonet] FW: The results of your email commands
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <FA607DC3D1ED7C46A9546820A3EB877F0A281A01 <@t> LBMSG02.hchd.local>
Content-Type: text/plain; charset="us-ascii"



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
Sent: Tuesday, November 05, 2013 8:48 AM
To: Scott, Allison D
Subject: The results of your email commands

The results of your email command are provided below. Attached is your original message.

- Results:
    Ignoring non-text/plain MIME parts

- Unprocessed:
    Sent: Tuesday, November 05, 2013 8:47 AM
    To: histonet-request <@t> lists.utsouthwestern.edu
    Subject: FW: FaxitronX-Ray digital imaging
    From: Scott, Allison D
    Sent: Tuesday, November 05, 2013 8:46 AM
    To: histonet-request <@t> lists.utsouthwestern.edu<mailto:histonet-request <@t> lists=
    .utsouthwestern.edu>
    Subject: Faxitron/X-Ray digital imaging
    Hello to all in histoland.  Our chief pathologist is interested in getting =
    a faxitron xray digital imaging machine.  Is anyone out there using this te=
    chnology and can you suggest a good company in which to order one from.  An=
    y help in this will be greatly appreciated.
    Allison Scott HT(ASCP)
    Supervisor, Histology Lab
    LBJ Hospital
    Harris Health System

- Ignored:
    Office: 713-566-2148
    Lab: 713-566-5287



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------------------------------

Message: 11
Date: Tue, 5 Nov 2013 10:08:46 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] Hot Histology Career Opportunities Alert from Pam
        Barker  at RELIA Solutions!! Even if you are happy where you are take   a
        look please we offer a 500.00 referral fee and it's almost      time for
        Christmas shopping!!! 11/5/2013
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00dc01ceda38$ecf3afa0$c6db0ee0$@earthlink.net>
Content-Type: text/plain;       charset="us-ascii"

Hi Histonetters!!
I hope you are off to the start of a great week on this beautiful Fall Day.
I wanted to drop you a line about some of the opportunities that I am
currently working on.
These clients aren't just "kicking tires" they are ready to interview and
ready to hire.  If you are looking to make a change now or make a commitment
now and the actual change after the holidays if you are the right person for
my client they will do what it takes to get you I guarantee it!!
So grab a cup of coffee or a mug of tea and something pumpkin flavored
(isn't everything pumpkin flavored this time of year?) and please take a
minute to peruse my list of current openings.
 All of the positions I work with are fulltime permanent positions with some
of the best facilities Nationwide.
My clients offer excellent compensation including competitive salaries,
great benefits, relocation assistance/sign on bonuses.
Here is a list of my current openings:

HISTOLOGY SUPERVISORS/MANAGERS: WA OH MA GA
Pathology Supervisor - Seattle, WA
Lead Pathology Tech - East of Columbus, OH
Histology Supervisor - Boston, MA
Histology Supervisor - Atlanta, GA

HISTOTECHNICIANS/HISTOTECHNOLOGISTS:  VA OH NM MA
Histology Tech - Roanoke, VA
Histotechnician Day shift -East of Columbus, OH
Grossing Histotechnician Dayshift - Las Cruces, NM
Histotechnician - Nights - Nashville, TN
Night Shift Grossing Histotech - Boston, MA

If you or any of your friends would like more information on any of these
positions or if you would like to discuss opportunities in other areas or
future job searches please contact me.  I would be more than happy to assist
you.  You can reach me at 866-607-3542 or relia1 <@t> earthlink.net

Remember I offer over 20 years of recruiting and employment counseling
experience, knowledgeable, confidential and responsive service to you and
your friends and a permanent placement practice dedicated to the histology
profession.

I work with facilities nationwide and I will keep your resume confidential.
I will only represent you to jobs you tell me you are interested in looking
into.

Remember. It never hurts to keep an eye open even if you are happy in your
present job.
Pam - 866-607-3542 (866-60RELIA)

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia







------------------------------

Message: 12
Date: Tue, 5 Nov 2013 10:17:26 -0500
From: "Richard Yeo" <ryeo <@t> foxmail.wchosp.org>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <17E893BEFAF44C0EAD05AF8876EDAF80.MAI <@t> foxmail.wchosp.org>
Content-Type: text/plain; charset="utf-8"


   I  have  an  abundance  of fungus controls. I need afb and h.pylori. I
   would be   me know an   Thanks
   Richard Yeo ryeo <@t> foxma

------------------------------

Message: 13
Date: Tue, 5 Nov 2013 16:00:11 +0000
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
Subject: [Histonet] Faxitron Xray digital imaging
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <FA607DC3D1ED7C46A9546820A3EB877F0A281A41 <@t> LBMSG02.hchd.local>
Content-Type: text/plain; charset="us-ascii"

Hello to all in histoland.  Our chief pathologist is interested in getting a faxitron xray digital imaging machine.  Is anyone out there using this technology and can you suggest a company in which to order one from.  Any help in this will be greatly appreciated.




Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287


CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
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To the extent the information in this e-mail and any attachments contain
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and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged.  This e-mail may also be confidential and/or privileged under
Texas law.  The e-mail is for the use of only the individual or entity named
above.  If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.



------------------------------

Message: 14
Date: Tue, 5 Nov 2013 09:47:16 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> outlook.com>
Subject: RE: [Histonet] Faxitron Xray digital imaging
To: "Scott, Allison D" <allison.scott <@t> harrishealth.org>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL129-W16EFC98ED28B25E187DEC182F10 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery.

There is only one company that manufactures and sells the instrument:
Faxitron Bioptics, LLC
Tucson, AZ
877-910-0030

The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation.
William DeSalvo, BS HTL(ASCP)


> From: Allison.Scott <@t> harrishealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 5 Nov 2013 16:00:11 +0000
> Subject: [Histonet] Faxitron Xray digital imaging
>
> Hello to all in histoland.  Our chief pathologist is interested in getting a faxitron xray digital imaging machine.  Is anyone out there using this technology and can you suggest a company in which to order one from.  Any help in this will be greatly appreciated.
>
>
>
>
> Allison Scott HT(ASCP)
> Supervisor, Histology Lab
> LBJ Hospital
> Harris Health System
> Office: 713-566-2148
> Lab: 713-566-5287
>
>
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from
> your computer system.
>
> To the extent the information in this e-mail and any attachments contain
> protected health information as defined by the Health Insurance Portability
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
> privileged.  This e-mail may also be confidential and/or privileged under
> Texas law.  The e-mail is for the use of only the individual or entity named
> above.  If you are not the intended recipient, or any authorized
> representative of the intended recipient, you are hereby notified that any
> review, dissemination or copying of this e-mail and its attachments is
> strictly prohibited.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

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End of Histonet Digest, Vol 120, Issue 6
****************************************



------------------------------

Message: 4
Date: Wed, 6 Nov 2013 05:37:13 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] FW: Fungus Controls
To: 'Tim Higgins' <thigginsht <@t> msn.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>, "Vickroy.Jim <@t> mhsil.com"
	<vickroy.jim <@t> mhsil.com>
Message-ID:
	<E9A90E28259D2F4E84308C5E8EA8F7B401694CAE1D35 <@t> lmhs-exchange>
Content-Type: text/plain; charset="us-ascii"

I made some controls years ago that we are still using today.  I used bread mold and streaked it out on a blood agar plate.  Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed.  Cuts and stains beautifully.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, November 05, 2013 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu; Vickroy.Jim <@t> mhsil.com
Subject: [Histonet] FW: Fungus Controls




I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains.  Its "naturally occurring", a petri dish would not be naturally occurring in my mind.

Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it.  Actually happy I was able to save some money.

Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!!  I have never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC


> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie <foreightl <@t> gmail.com>
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>       <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>       <CAKEyg-2KaDkn0oFQURzuPcugQt=Ju8LKK6C8daH+nPjvVsPMJw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Jim,
>
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
>
> Good luck!
>
>
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:
>
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
                                          _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.



------------------------------

Message: 5
Date: Wed, 06 Nov 2013 17:33:59 +0500
From: tahseen <tahseen <@t> brain.net.pk>
Subject: [Histonet] CD 34 antibody abcam (ab963)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <b3869a2ddbd2df0fe67fec3b7ae6d12c <@t> brain.net.pk>
Content-Type: text/plain; charset=UTF-8; format=flowed

Hi All,
We used CD 34 antibody abcam (ab963)with detection kit enVision to 
stain our samples from rats and rabbits. We need to stain vessels only 
but macrophages, fibroblast, collagen all are stained in other hand 
known +ve control (Tonsil) is perform good.  Please help us that what 
should we do for IHC staining of our samples.
Best regards
Muhammad Tahseen
Sr.Supervisor Histology
SKMCH&RC
Lahore




------------------------------

Message: 6
Date: Wed, 6 Nov 2013 15:38:53 +0000
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Water rinse for H&E's
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F8593D93099A864A9730359FA6992A874750A388 <@t> CHIEX015.CHI.catholichealth.net>
	
Content-Type: text/plain; charset="us-ascii"

Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_pardue <@t> memorial.org

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.


------------------------------

Message: 7
Date: Wed, 6 Nov 2013 07:50:32 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Water rinse for H&E's
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1383753032.32943.YahooMailNeo <@t> web163103.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I do not, but it seems that you need to use hot water. If it works for you, keep doing it.
?
The rationale behind this issue is that water after the staining is not just waterto wash the sections, but also to change the pH of the hematoxylin so the stain is, lets say, "developed" and this is a process temperature driven (the speed of the reaction is directly proportional to the temp.) and if you are having weak results with your running water (evidently cold) and good with hot water, this demonstrates that you need hot water in your specific circumstances.
I hope I explained myself
Ren? J.


________________________________
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, November 6, 2013 10:38 AM
Subject: [Histonet] Water rinse for H&E's


Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_pardue <@t> memorial.org

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Wed, 6 Nov 2013 12:20:48 -0500
From: "Terri Brown" <Terri.Brown <@t> Northside.com>
Subject: RE: [Histonet] Water rinse for H&E's
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<731941C266951A47BEF11E5EFAAED9C91EF5D2F4 <@t> nsmvexch01.northside.local>
Content-Type: text/plain;charset="us-ascii"

We use warm water rinses for our H&E slides on our automatic stainers.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.brown <@t> northside.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pardue,
Judith
Sent: Wednesday, November 06, 2013 10:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Water rinse for H&E's

Does anyone use hot water rinses on their h&e slides. If we use cold
water our slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_pardue <@t> memorial.org

This electronic mail and any attached documents are intended solely for
the named addressee(s) and contain confidential information. If you are
not an addressee, or responsible for delivering this email to an
addressee, you have received this email in error and are notified that
reading, copying, or disclosing this email is prohibited. If you
received this email in error, immediately reply to the sender and delete
the message completely from your computer system.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.




------------------------------

Message: 9
Date: Wed, 6 Nov 2013 17:22:14 +0000
From: Joanne Clark <jclark <@t> pcnm.com>
Subject: [Histonet] Biopsy Problems
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there.  After staining, when looking at it on the scope, you can't get it to focus in one plane.  It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section.  The cells, when in focus, look fixed and properly processed, and the staining is the way it should be.  I have no idea what is causing this artifact.  Has anyone else seen this and can shed some light on what is causing it?

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico




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