[Histonet] Bone Marrow Issues

[C.D.G.]

Shiley: positive charged slides do not mantain certain tissues on them well. Blood is one of them,
and it is advisable to mount your bloody bone marrows on slides treated with vinyl glue, like Elmer's or 
the one you prefer.
My regards,
Carlos.

  *********** REPLY SEPARATOR  ***********

On 29/05/2013 at 11:31 a.m. Shirley A. Powell wrote:

>I had this happen when I was doing IHC and changed to regular slides and
>used StayOn from Leica. Had more success with keeping the tissue on the
>slides.  I now do autopsy slides and do the same.
>
>Shirley
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jordan
>Phillips
>Sent: Tuesday, May 28, 2013 7:56 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Bone Marrow Issues
>
>HI everyone.  We are currently having problems with all of our bone
>marrows, mainly clots, washing off the slides.  We fix our bone marrows in
>Zenkers.  We use positive charged slides, put the slides in the oven for a
>minimum of 2 hours and hand depar with no agitation.  When we get to the
>water, the sections just float off the slides, leaving just a rim of the
>tissue.   Any tips or suggestions would be greatly appreciated! 
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