[Histonet] another Movats question (paraffin)

[Lee & Peggy Wenk]

What happens if you skip the ammonium hydroxide?

Peggy Wenk
-----Original Message----- 
From: Molinari, Betsy
Sent: Wednesday, May 29, 2013 7:45 AM
To: Lee & Peggy Wenk ; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] another Movats question (paraffin)

Hi Peggy,
No, this is a batch I have been using since 12/12. I purchased it from 
Sigma. It seems faint after the ammonium hydroxide . I did a run yesterday 
and I did get some blue, but faintly, not as bright as I am used to seeing. 
Everything else was picture perfect. Ray suggested that it may be a change 
in my tap water. I have our current report and am waiting on the report 
before that one. I am doing another run today and am considering rinsing in 
DH2O after the Alcian Blue and ammonium hydroxide.
What do you think?
Betsy



-----------------------------


Betsy  Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org | www.texasheart.org

6770 Bertner Ave., MC 1-283, Houston, TX 77030



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-----Original Message-----
From: Lee & Peggy Wenk [mailto:lpwenk <@t> sbcglobal.net]
Sent: Tuesday, May 28, 2013 6:40 PM
To: Molinari, Betsy
Subject: Re: [Histonet] another Movats question (paraffin)

Any chance this is a new batch of alcian blue? From where did you purchase 
it? I have a theory, but would like some more information first.

Peggy A. Wenk, HTL(ASCP)SLS

Lee & Peggy Wenk
-----Original Message-----
From: Molinari, Betsy
Sent: Tuesday, May 28, 2013 11:47 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] another Movats question (paraffin)

Hi,
The Alcian Blue in my Movats  is very faint or seems to completely 
disappear. I have been running Movats for years and this is a new problem. I 
have checked lot #’s everything is the same. The solution is 1% pH 2.7. The 
slides are in solution for 20 mins, rinse for 5 mins in tap H2O,  then in 
alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed 
for 10 mins. But after hematoxylin the blue is either gone or very faint.
Any suggestions?
Thanks,
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston , TX 77030
832-355-6524 (lab)
832-355-6812 (fax)



<http://www.texasheart.org>



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org> | 
www.texasheart.org<http://www.texasheart.org>



6770 Bertner Ave., MC 1-283, Houston, TX 77030



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