[Histonet] Re: Histonet Digest, Vol 114, Issue 8

Tyrone Genade tgenade <@t> gmail.com
Fri May 10 01:56:43 CDT 2013

On Wed, 8 May 2013 19:25:21,  "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu
> wrote:

> Our S100 is not working very well.  I think it's too old.  Can someone
recommend an S100
> antibody that works well in dog?  It would be really nice to find a good
monoclonal instead
> of the polyclonals.
> We are looking for schwannomas,  astrogliomas, malignant melanomas and
> metastases.

How are you processing your tissue? S100 is very soluble and can wash out
of unfixed frozen sections. I only achieve stating, using the DAKO
anti-S100 polyclonal, if I thoroughly fix my brains first in PFA.

The DAKO anti-S100 is a diagnostic antibody so it should give no problems
unless, as you suspect, it is very old. But then it has to be VERY old as I
am using antibody aliquots which have been stored at -20 oC that are now 6
years old and regularly retrieve antibodies from -80 that have been there
for more than 20 years. If the antibody has been stored at 4 oC then it
could be past its usefulness... But even then, many are very stable and can
last years.

Kind regards
Tyrone Genade Ph.D.
Department of Human Biology
University of Cape Town
email: tgenade <@t> gmail.com
tel: +27-84-632-1925 (c)
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