[Histonet] linear stainer hematoxylin staining issues

[arjun ruhella]

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Thank you,
Arjun

On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari <kkienitz <@t> orclinic.com>wrote:

> If your acetic acid solution is made in-house....I would suggest remaking
> it, just in case it was accidently made stronger.
>
>
> Kari Kienitz HT, (ASCP)
> Histology Laboratory
> Portland Gastroenterology
> The Oregon Clinic
> 1111 NE 99th Ave
> Portland, OR  97220
> 503.935.8311
> kkienitz <@t> orclinic.com
>
>
>
>
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> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sophia Lin [
> lins0701 <@t> gmail.com]
> Sent: Wednesday, March 27, 2013 9:26 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] linear stainer hematoxylin staining issues
>
> We have the Hacker linear stainer, and for the past two weeks we had clean,
> crisp H&E staining. However, last Friday, something happened and our H&E
> stains no longer look crisp, but instead have a hazy, unreadable look.
> Nothing different has been done, and we have troubleshot for every possible
> scenario. After a clean set up, we noticed that the hematoxylin is not
> staining very well. We have it set for 2 minutes with agitation for a
> uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and
> the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for
> total of 4.5 minutes), we use 1.5% acetic acid. It seems that the
> hematoxylin is not staining well.
>
> Any tips? We may need to consider increasing a bucket for hematoxylin?
>
> Thanks,
>
> Sophia
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