[Histonet] Re: hematoxylin back to deparaffin?

[Teri Johnson]

Hi Sophia,

If you plan to do immunostains that will eventually be hematoxylin counterstained, just let it be. If you need them to be completely decolorized, put them in 0.5% periodic acid and the rest of it should come out. I would be cautious however with what you do to the sections. It might make a difference to any subsequent testing downstream. Make sure you use two sets of controls, one with just regular depar, and one treated just like these slides.

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752