[Histonet] Mallory triple stain

[Massimo]

Hi all,

you can find the original Mallory's trichrome stain with a typical example
about the preparation of a transverse section of Amphioxus using the acid fuchsin-anilin 
blue-orange G stain of Mallory (1901) at this address:

http://www.mediafire.com/view/?ca96bvrdmp22te6


That's an abstract from the "A Text-book of Histology", please see at:

http://www.mediafire.com/view/?5gjm10epdg303ja


Kind Regards,

Massimo Tosi
"A humble
Chemical Engineer who loves Histology"




________________________________
 Da: Rene J Buesa <rjbuesa <@t> yahoo.com>
A: "pruegg <@t> ihctech.net" <pruegg <@t> ihctech.net>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Inviato: Venerdì 22 Marzo 2013 14:24
Oggetto: Re: [Histonet] Mallory triple stain
 
Not really! There are scores of trichrome stains and Mallory is one of them with the characteristic that cell nuclei are not stained with hematoxylin, while in Masson TC they are. 
I personally prefer Mallory, it is more color "rich" and simpler to do. It was invented at the end of the XIX century and Masson's in the early 1930's
With TC stains the histologist's preferences rule, even when all stain the same but with different colors and intensities. 
René J.

From: "pruegg <@t> ihctech.net" <pruegg <@t> ihctech.net>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, March 21, 2013 5:58 PM
Subject: [Histonet] Mallory triple stain

Does anyone know what this is about?  I think these people just want a
trichrome stain and we are already doing a Masson's TC for them using
aniline blue.  I think they are just asking for a modified version of TC and
want to make sure we use aniline blue for the collagen stain???  Is Mallory
Triple stain something other than a modified trichrome stain?





"What we were asking for was Mallory Triple stain, which appears to be the
synonymous with Mallory Aniline Blue, and Mallory Trichrome. Is that
correct, that those three names are for the same staining technique? The
main components of the stain that we were looking for were: aniline blue,
orange G, and acid fuchsin. As far as I can tell all three techniques have
those three components."





Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting

40864 E. Arkansas Ave

Bennett, CO 80102

H 303-644-4538

C 720-281-5406

<mailto:rueggihcconsultingpr <@t> outlook.com> rueggihcconsultingpr <@t> outlook.com








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