[Histonet] Thionin / Acid fuchsin staining Rat brain

[Mohit Koladia]

*Hello Histonetters,*
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*I am looking for the staining protocol for thionin/Acid fucshin staining.*
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*I have Rat brain slides which are paraffin embedded and sectioned at 5-7
um. *
*I have had a problem to make a Acid fucshin solution as it does not stick
to the brain slices.*
*Please give proper recipe for the same.*
*I tried to search a lot but i didnt find any good article or literature
that helps figuring this out.*
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*Would appreciate if someone can help me in this regards.*
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*Thanks,*
*
Mohit Koladia (B.Pharm, MS Pharm)
Graduate Research Scholar,
North Dakota State University,
Fargo, ND, USA-58102*