[Histonet] Possible continuing automatic staining problem

[Bartlett, Jeanine (CDC/OID/NCEZID)]

Are you talking bout H&E's? If so what hematoxylin and eosin are you using? What protocol?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lisa
Sent: Saturday, March 16, 2013 1:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Possible continuing automatic staining problem 

Hi
We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope......Please any suggestions out there, Dr & histotech's very frustrated.
Lisa A

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