[Histonet] IHC validation

[WILLIAM DESALVO]

Laurie, I would use the control slides to validate and set up the antibodies. I good secondary check/control would be to send slides to another lab to check for correlation and confirmation of your protocol performance. Once you get an established process, then you can later check patient samples to the protocol and make adjustments, as necessary.

William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

 

> From: lcolbert <@t> pathmdlabs.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 15 Mar 2013 16:51:27 +0000
> Subject: [Histonet] IHC validation
> 
> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?
> 
> Laurie Colbert, HT (ASCP)
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