[Histonet] RE: IHC validation

[Morken, Timothy]

Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok.

The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing. 

Tim Morken
Department of Pathology
UC San Francisco Medical Center


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, March 15, 2013 9:51 AM
To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] IHC validation

If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?

Laurie Colbert, HT (ASCP)
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