[Histonet] RE: AFB and negative control

[Cristi Rigazio]

Isn't a gram stain the only special that requires a positive and negative control?  

Sent from my iPhone

On Mar 14, 2013, at 1:51 PM, joelle weaver <joelleweaver <@t> hotmail.com> wrote:

> This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. 
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> Joelle Weaver MAOM, HTL (ASCP) QIHC
>> From: TNMayer <@t> mdanderson.org
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Thu, 14 Mar 2013 17:52:39 +0000
>> Subject: [Histonet] RE: AFB and negative control
>> 
>> While I don't use a negative control for the AFB, I will use distilled water throughout the procedure.  Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well.  Make sure the waterbath has been disinfected.
>> 
>> Toysha N. Mayer, MBA, HT (ASCP)
>> Instructor, Education Coordinator
>> Program in Histotechnology
>> School of Health Professions
>> MD Anderson Cancer Center
>> (713) 563-3481
>> tnmayer <@t> mdanderson.org
>> 
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>> 
>> Date: Thu, 14 Mar 2013 12:42:30 +0000
>> From: Ian R Bernard <ibernard <@t> uab.edu>
>> Subject: [Histonet] AFB and Negative Control
>> To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>, "Tighe, Sean T"
>>    <stighe <@t> ufl.edu>,    "histonet <@t> lists.utsouthwestern.edu"
>>    <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>    <D4F4C602B10B9F45B4E9271AF6380E16181A1114 <@t> UABEXMB1.ad.uab.edu>
>> Content-Type: text/plain; charset="utf-8"
>> 
>> The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now.  
>> 
>> Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well?  Any suggestion on a good control tissue type? Carson recommends uterus.  Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this?
>> 
>> Any thoughts from fellow histonetters?
>> 
>> Thanks
>> Ian Bernard
>> 
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