[Histonet] SMC Immuno and Elastin combo?

[Jan Shivers]

Patti,

You're using a mouse monoclonal antibody on mouse tissue, so be aware that
the secondary link antibody will bind to any mouse IgG it finds in the
tissue (normal plasma cells, IgG in normal sera present inside blood
vessels, or as infiltrating interstitial normal sera, etc.), not just to
the primary anti-Actin antibody.  You may have to use a mouse-on-mouse kit
for the Actin staining portion, if you see too much nonspecific background
in your tissue.

Jan Shivers
UMN Vet Diag Lab

On Wed, Mar 13, 2013 at 10:58 AM, Patricia Bourassa <
pbourassa <@t> karospharma.com> wrote:

> Hello,
>
> I've read some papers which reference the combination of smooth muscle cell
> actin immunostaining with a Verhoeff's elastin counterstain.
>
> Has anyone ever done this and if so, do you have protocol you'd be willing
> to share?
>
> I'm staining mouse and rat lung and have a SMC protocol working that uses
> an Abcam antibody and NovaRed chromagen (peroxidase).   I've also done EVG
> staining so I'm familiar with the general process.  The combination is what
> I'm not sure of - I gave it a try for the heck of it and wiped out the
> immunostain.
>
> We've just started looking at this, so nothing is set in stone and I'd be
> grateful for any advice/ procedural help.
>
> Thanks in advance!
>
> Patti Bourassa
> Karos Pharmaceuticals
> --
> *Patti Bourassa
> Senior Scientist
> Karos Pharmaceuticals
> (203) 535-0540, ext 207*
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>